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Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR 55
Santos,Cristina; Teixeira,Fabiane; Vicente,Ana; Astolfi-Filho,Spartaco.
Chlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who sought treatment for STD in the Alfredo da Matta Institute of Dermatology and Venerology and the Institute of Tropical Medicine of Amazonas in Manaus, Brazil. These women were examined by a specific PCR for the chlamydial plasmid, and the nature of the amplicon was determined by restriction analysis and DNA sequencing. The PCR diagnosis revealed a prevalence of 20.7% infected women.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlamydia trachomatis; PCR; DST.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200001
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BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG¯CTC-3' 58
Chies,Jocelei M.; Dias,Ana C. de O.; Maia,Hélio M.M.; Astolfi-Filho,Spartaco.
A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG<FONT FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Type II restriction endonuclease; BpuAmI; SacI; Neoschizomers; Bacillus pumilus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100018
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Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis 58
Chies,Jocelei Maria; Dias,Ana Christina de Oliveira; Maia,Hélio Mauro Moreira; Braga,Luis Paulo da Silva; Astolfi-Filho,Spartaco.
The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Type II restriction endonuclease; BliAI; ClaI; Isoschizomers; Bacillus licheniformis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400027
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Conservation implications of the mating system of the Pampa Hermosa landrace of peach palm analyzed with microsatellite markers 74
Picanço-Rodrigues,Doriane; Astolfi-Filho,Spartaco; Lemes,Maristerra R.; Gribel,Rogerio; Sebbenn,Alexandre M.; Clement,Charles R..
Peach palm (Bactris gasipaes) is cultivated by many indigenous and traditional communities from Amazonia to Central America for its edible fruits, and is currently important for its heart-of-palm. The objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. Eight microsatellite loci were used to genotype 24 open-pollinated progenies from three populations of the Pampa Hermosa landrace maintained in a progeny trial for genetic improvement. Both the multi-locus outcrossing rates (0.95 to 0.99) and the progeny level multi-locus outcrossing rates (0.9 to 1.0) were high, indicating that peach palm is predominantly allogamous. The outcrossing rates among relatives were significantly different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bactris gasipaes; Bi-parental inbreeding; Genetic improvement; Genetic conservation; Outcrossing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100059
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