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| Arcos-Ortega,Guadalupe Fabiola; Chan-Kuuk,Rafael Antonio; González-Kantún,Wilma Aracely; Souza-Perera,Ramón; Nakazawa-Ueji,Yumi Elena; Avilés-Berzunza,Elidé; Godoy-Hernández,Gregorio; Lawton,Michael A; Zúñiga Aguilar,José Juan. |
| Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β-glucuronidase activity. The standardized protocol, which yielded nearly 55%... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; GUS assay. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400007 |
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