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Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France ArchiMer
Azandegbe, Afi; Gamier, Matthieu; Andrieux, Francoise; Kerouel, Roger; Philippon, Xavier; Nicolas, Jean-louis.
Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V. aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V....
Tipo: Text Palavras-chave: Vibrio aestuarianus; Crassostrea gigas Sediment; Seasonal abundance; Colony-blot.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12813/9756.pdf
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Influence of oyster culture on biogeochemistry and bacterial community structure at the sediment-water interface ArchiMer
Azandegbe, Afi; Poly, Franck; Andrieux, Francoise; Kerouel, Roger; Philippon, Xavier; Nicolas, Jean-louis.
Bacterial community structure and some biogeochemical parameters were studied in the sediment of two Pacific oyster farming sites, Aber Benoît (AB) and Rivière d'Auray (RA) in Brittany (France), to examine the ecological impact of oysters and to evaluate the emission of sulfide and ammonia from sediment. At AB, the organic matter accumulated in the sediment beneath the oyster tables was rapidly mineralized, with strong fluxes of ammonia and sulfide that reached 1014 μmol m−2 h−1 - and 215 μmol m−2 h−1, respectively, in June 2007. At RA, the fluxes were about half as strong on average and better distributed through the year. The ammonia and sulfide concentrations in the overlying water never reached levels that would be toxic to oysters in either site, nor...
Tipo: Text Palavras-chave: Sediment; Biogeochemical parameters; Fluxes; Bacterial structure; Crassostrea gigas.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19010/17083.pdf
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Impact of Oyster Farming on Diagenetic Processes and the Phosphorus Cycle in Two Estuaries (Britanny, France) ArchiMer
Andrieux, Francoise; Azandegbe, Afi; Caradec, Florian; Philippon, Xavier; Kerouel, Roger; Youenou, Agnes; Nicolas, Jean-louis.
This study aims to compare the impact of oyster cultures on diagenetic processes and the phosphorus cycle in the sediments of the Aber Benoît and the Rivière d’Auray, estuary of Brittany, France. Our results showed clear evidence of the seasonal impact of oyster cultures on sediment characteristics (grain size, organic matter parameters), and the phosphorus cycle, especially in the Aber Benoît. At this site, seasonal variations of sulfide and Fe concentrations in pore waters, as well as Fe-P concentrations in the solid-phase highlighted a shift from a system governed by iron reduction (Reference) to a system governed by sulphate reduction (Beneath Oyster). This could be partly explained by the increase in labile organic matter (ie, biodeposits) beneath...
Tipo: Text Palavras-chave: Sediment; Phosphorus; Sulfide; Dissolved Fe/P ratios; Labile organic matter; Oyster cultures.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00209/32003/30441.pdf
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Etude de la structure des communautés bactériennes du sédiment et de l'écologie de Vibrio aestuarianus pathogène de l'huître creuse Crassostrea gigas dans deux sites ostréicoles ArchiMer
Azandegbe, Afi.
Since several years, the French oyster farming copes with high mortality affecting juveniles and some adults of the Pacific oyster Crassostrea gigas. Studies conducted in the framework of a multidisciplinary project (MOREST) showed that mortality was related to several factors like physiological and genetic status of oyster, pathogens including bacteria of the genus Vibrio, and revealed that the sediment, on which the oyster cages are installed, might play a particular role. This has been suggested because of the concomitance of the mortalities and increased toxic compounds like ammonia and sulphides in the sediment. Moreover, the presence of Vibrio bacteria in the sediment indicates a dual role of sediment in the mortality onset. The physico-chemical...
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarianus; Écologie; Sédiment; Ammonium; Sulfures; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Ecology; Sediment; Ammonia; Sulphide.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00032/14277/11561.pdf
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