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First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? ArchiMer
Prado-alvarez, Maria; García-fernández, Pablo; Faury, Nicole; Azevedo, Carlos; Morga, Benjamin; Gestal, Camino.
The ostreid herpes virus (OsHV-1), associated with massive mortalities in the bivalve Crassostrea gigas, was detected for the first time in the cephalopod Octopus vulgaris. Wild adult animals from a natural breeding area in Spain showed an overall prevalence of detection of 87.5% between 2010 and 2015 suggesting an environmental source of viral material uptake. Overall positive PCR detections were significantly higher in adult animals (p = 0.031) compared to newly hatched paralarvae (62%). Prevalence in embryos reached 65%. Sequencing of positive amplicons revealed a match with the variant OsHV-1 µVar showing the genomic features that distinguish this variant in the ORF4. Gill tissues from adult animals were also processed for in situ hybridization and...
Tipo: Text Palavras-chave: Octopus vulgaris; Crassostrea gigas; OsHV-1; Gene expression; In situ hybridization.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00680/79201/81700.pdf
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