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| Espinosa,Ivette; Báez,M; Percedo,María Irian; Martínez,Siomara. |
| Streptococcus suis is a gram-positive bacterium that causes serious diseases in pigs and in humans with occupational risk. The DNA extraction methods for amplification of gene fragments by PCR for typing S.suis may be complex, and expensive chemical reagents and time consuming. The aim of this study was to evaluate a method for the rapid release of the genomic DNA from S.suis colonies by using a physical method based on heating and freezing; in this case, temperatures of 100ºC and 95ºC were tested. The results showed that DNA extraction directly from colonies by heating at 100ºC could be useful for an easy genotyping of S.suis strains in a short time, while 95ºC was not sufficient for DNA release. The detection limit of the PCR assay using DNA obtained by... |
| Tipo: Journal article |
Palavras-chave: Streptococcus suis; Direct colony PCR. |
| Ano: 2013 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100009 |
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| Espinosa,Ivette; Báez,M; Vichi,J; Martínez,Siomara. |
| Pasteurella multocida is an important veterinary pathogen that causes a range of animal diseases, including fowl cholera, hemorrhagic septicemia in cattle and atrophic rhinitis in swine. P. multocida is generally recognized as a secondary invader contributing substantially to respiratory diseases in pigs by aggravation of lung lesions. Five capsule serogroups are routinely identified in P. multocida (A, B, D, E, and F) and each is generally associated with, but not completely restricted, to a specific host. A total of 16 isolates of Pasteurella multocida capsular genotype A and two isolates capsular genotype D were characterized for their susceptibilities to 10 antibiotics and the presence of four genes for virulence factors associated to adherence. The... |
| Tipo: Journal article |
Palavras-chave: P. multocida; Virulence factor; PCR; Antimicrobial susceptibility. |
| Ano: 2012 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000300003 |
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| Espinosa,Ivette; Colas,M; Vichi,Joivier; Báez,M; Martínez,Siomara. |
| Respiratory infection is the most serious disease affecting poultry and causing great economic losses to the poultry industry worldwide. In avian host, several microorganisms of the genus Pasteurella (P. multocida, P. gallinarum and P. anatipestifer), Bordetella (B. avium) and Avibacterium paragallinarum) were involved in respiratory diseases complex. Ornithobacterium rhinotracheale is a recently discovered bacterium, of worldwide distribution in commercial poultry, in which it is associated to respiratory diseases and it is also found in wild birds. Airsacculitis and pneumonia are the most common symptoms of infection with O. rhinotracheale. Isolation and identification of pathogenic bacteria associated to respiratory diseases were carried out from a... |
| Tipo: Journal article |
Palavras-chave: Pasteurella multocida; Escherichia coli; Ornithobacterium rhinotracheale; Layer hens. |
| Ano: 2011 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100006 |
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