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Growth of in vitro Fusarium oxysporum f. sp. niveum in chemically defined media amended with gallic acid Biol. Res.
WU,HONG-SHENG; WANG,YANG; ZHANG,CHAO-YONG; BAO,WEI; LING,NING; LIU,DONG-YANG; SHEN,QI-RONG.
Gallic acid was artificially added to the media to grow Fusarium oxysporum f.sp.niveum to investigate its effect on the pathogenic fungus. Results indicate that gallic acid inhibited the growth of F. oxysporum f.sp.niveum. The colony diameter, the conidia germinating rate and the conidia yield were reduced by 5.7-22.9%%, 35.8-55.6% and 38.9-62.2% respectively. However, the virulence factors by the fungus were stimulated. The activity of pectinase, proteinase and cellulase increased by 12.3-627.8%, 11.8-41.2% and 0.5-325.0% respectively, while the activity of amylase increased slightly. The results suggest that gallic acid repressed growth but facilitated the relative pathogenicity of invading pathogens.
Tipo: Journal article Palavras-chave: Allelopathy; Gallic acid; Fusarium oxysporum f. sp. niveum; Plant-microbe interaction; Virulent factors.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300004
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The proapoptotic activity of C-terminal domain of apoptosis-inducing factor (AIF) is separated from its N-terminal Biol. Res.
ZHANG,YONG; HAN,TAO; ZHU,QING; ZHANG,WEI; BAO,WEI; FU,HAI-JING; YANG,JIE; HUANG,XIAO-JUN; WEI,JUN-XIA; MENG,YAN-LING; ZHAO,JING; CAO,YUN-XIN; JIA,LIN-TAO; YANG,AN-GANG.
Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein that mediates both NADH-oxidizing and caspase-independent apoptosis. Further, the proapoptotic activity of AIF is located in the C-terminus of AIF, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. In the present study, we generated two truncated AIFs, AIFΔ1-480-FLAG, which is a FLAG-tagged C-terminal peptide comprising amino acids from 481 to 613, and AIF360-480 containing amino acids from 360 to 480 of AIF. We used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. AIFΔ1-480 but not AIF360-480 induces apoptosis in transfected cells. We also...
Tipo: Journal article Palavras-chave: Apoptosis inducing factor (AIF); Apoptosis; Cytochrome c; Mitochondria; HER2.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200014
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