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SANTOS,Renato de Lima; BARRETO FILHO,João Bosco; MARQUES Jr.,Antônio de Pinho; ANDRADE,Joelmer Santos. |
The goal of this research was to determine the volumetric proportions of the structural components of the goat placenta throughout gestation. We used nine goats of nondefined breed, separated into groups A, B and C, with 90, 120 and 150 days of gestation, respectively. From each animal fragments of three placentomes were obtained, one removed from the base, another from the middle part and the other from the end of the gestational uterine horn. The fragments were fixed in Bouin solution and histologically processed according to the standard techniques and stained with hematoxilin-eosin. Microscopically, a 25-point circular grid (Zeiss KPL ocular 6.3x with 25 points) was randomly used across the placentome to determine the volumetric proportion of the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Placenta; Morphology; Goat. |
Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000400002 |
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EIRAS,Paola Ribeiro Seabra; BARRETO FILHO,João Bosco; GOLGHER,Romain Rolland; SANTOS,Sandra Regina Quintino dos. |
Bovine karyotyping has become an important diagnostic tool in animal breeding. In the prenatal period it can diagnose several chromosomal abnormalities such as Robertsonian translocations, testicle feminization syndrome, gonadal dysgenesis and Klinefelters syndrome. An important cell source for karyotype analysis is the amniotic fluid. It has been extensively used in humans but in bovine, however, this is not the case despite its diagnostic value. Since a small percentage of cells is viable, cells and their growth conditions as well as the handling of the material should be optimal to insure a successful analysis. For this, we have compared the growth efficiency for bovine amniocytes in two media, employing cells from 10 to 14 weeks of gestation.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotypes; Cell culture; Amnion; Cattle. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400005 |
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