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| FRANCO,OCTÁVIO LUIZ; GONDIM,LORRANCE ABREU; BEZERRA,KÁTIA REGINA; GUERRA,MARIA ELANE DE CARVALHO; LIMA,CARMEM ROGÉLIA FARIAS MACHADO; ENÉAS-FILHO,JOAQUIM; PRISCO,JOSÉ TARQUÍNIO; GOMES-FILHO,ENÉAS. |
| Partial purification and characterization of ribonucleases (RNase; EC 3.1.27.1) present in roots, stem and leaves of 5 day-old Pitiúba cowpea [Vigna unguiculata (L.) Walp.] seedlings are described. Crude extracts from the different tissues were precipitated with ammonium sulfate followed by ionic exchange chromatography (CM-Cellulose) resulting in purification factors of 48-fold for roots, 21 for stem and 42 for leaves. No deoxyribonuclease activity was practically observed. The molecular masses of the RNases did not significantly differ, averaging 16.3 kDa. Leaf RNase was stable up to 50ºC while the others were inactivated at this temperature. The maximal inactivation for both stem and roots RNases was reached at 70ºC while for leaf it occurred at 80ºC.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme effectors; Molecular mass; Nuclease; Optimum pH; Seedlings; Thermostability; Vigna unguiculata. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300010 |
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