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Registros recuperados: 12
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Sperathe effects of solid-state fermentation in the functional properties of defatted rice bran and wheat bran BABT
Silveira,Cristina Moreira da; Badiale-Furlong,Eliana.
Functional properties of fermented bran produced by Aspergillus oryzae and Rhizopus sp. in a solid-state fermentation system were determined, with an aim to evaluate their application in food formulation. The defatted rice bran and wheat bran were inoculated with the spores of the cultures and incubated at 30º C for 72 h. Samples were withdrawn at 0, 24, 48 and 72 h. Protein content, protein solubility, in-vitro digestibility, gelation and water holding capacity were determined in bran with or without fermentation. Rhizopus sp. increased significantly the protein content (69.0 and 56.0%, respectively, for defatted rice bran and wheat bran); protein solubility (28.5 and 36.2) and water holding capacity (11.4% for wheat bran). When A. oryzae was used all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Functional properties; Solid-state fermentation; Defatted rice bran; Wheat bran.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600027
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Solid-state fermentation for the enrichment and extraction of proteins and antioxidant compounds in rice bran by Rhizopus oryzae BABT
Kupski,Larine; Cipolatti,Eliane; Rocha,Meritaine da; Oliveira,Melissa dos Santos; Souza-Soares,Leonor de Almeida; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the solid-state fermentation with Rhizopus oryzae CCT 7560 of rice bran for the enrichment of proteins and the antioxidant compounds in the fermented biomass. Fermentation was performed in tray bioreactors at 30ºC for 120 h. Protein extraction was done at alkaline pH, followed by precipitation with acetone. Phenolic compounds were extracted with cold methanol. The maximum protein was recovered from after 120 h (26.6%). The content of total phenolic compounds increased during the fermentation and was maximum after 96 h, which inhibited the DPPH radical by 87%. The promising characteristics of the protein and phenolic extracts of the biomass suggested the application in the coating composition for vegetal tissues...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein extract; Phenolic extract; Fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600018
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Effect of deoxynivalenol and T-2 toxin in malt amylase activity BABT
Garda-Buffon,Jaqueline; Baraj,Edlira; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the β-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aminolitic enzymes; Fermentation; Trichothecenes; Barley.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300002
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Extraction of &#947-oryzanol from rice bran Ciência e Agrotecnologia
Heidtmann-Bemvenuti,Renata; Nora,Náthali Saião; Badiale-Furlong,Eliana.
γ-oryzanol, a phytochemical, has antioxidant activities and potential health benefits. In this study, we aimed to develop a method for extracting high amounts of γ-oryzanol from rice bran and for verifying its stability in different solvents. Of the 5 methods under investigation, the one that yielded the highest γ-oryzanol content was further improved by using a central composite rotational design (DCCR - 2(4), including 8 tests in axial conditions and 4 central points, totaling 28 trials). The parameters under study were temperature, time, volume, and the ratio of hexane:isopropanol. High γ-oryzanol content (13.98 mg.g-1) was obtained by using the following extraction conditions: 40° C, 40 min, and 75 mL hexane:isopropanol (1:3). The limits of detection...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Spectrophotometer; Stability; Phytochemistry.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600008
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Assessment of the antifungal activity of Spirulina platensis phenolic extract against Aspergillus flavus Ciência e Agrotecnologia
Souza,Michele Moraes de; Prietto,Luciana; Ribeiro,Anelise Christ; Souza,Taiana Denardi de; Badiale-Furlong,Eliana.
The production of safe food has stimulated the search for natural substances that possess antifungal activity. The indirect methods of estimating fungal biomass are based on the measurement of glucosamine, ergosterol and protein - typical compounds produced during the development of biomass. The aim of the study was to assess the effect of the phenolic extract from Spirulina platensis on the production of structural compounds in Aspergillus flavus, in order to identify its action on fungal inhibition. The Spirulina platensis methanolic extracts presented 1.15 mg phenolic compound/g Spirulina platensis, which showed an antifungal effect against Aspergillus flavus, inhibiting the glucosamine production up to 56%. Therefore, it may be employed as natural...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenolic inhibitors; Antifungal property; Glucosamine; Protein; Ergosterol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000600003
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Determinação de tricotecenos em cerveja e avaliação de incidência no produto comercializado no Rio Grande do Sul Ciênc. Tecnol. Aliment.
Garda,Jaqueline; Macedo,Rejane Martins; Badiale-Furlong,Eliana.
Este trabalho adaptou um método e avaliou os seus indicadores de mérito para determinação de tricotecenos, desoxinivalenol (DON) e toxina T-2, em cervejas comercializadas na região Sul do Rio Grande do Sul, empregando cromatografia gasosa. As adaptações foram realizadas na etapa de extração, purificação, derivação e condições cromatográficas usando amostras de cerveja artificialmente contaminadas, avaliando a adequação do procedimento pela recuperação obtida. Foram estudados os solventes acetonitrila, metanol e cloreto de metileno puro ou em misturas para a etapa de extração. A purificação do extrato foi realizada empregando ou não minicoluna de carvão:alumina:celite (7:5:3). Para a derivação testou-se basicidade do meio (bicarbonato de sódio, piridina),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tricotecenos; Cervejas; Desoxinivalenol; Toxina T-2; Cromatografia gasosa.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612004000400030
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Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp Ciênc. Tecnol. Aliment.
Pagnussatt,Fernanda Arnhold; Kupski,Larine; Darley,Francine Toralles; Filoda,Paula Freitas; Ponte,Émerson Medeiros Del; Garda-Buffon,Jaqueline; Badiale-Furlong,Eliana.
The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract was assessed for its antifungal activity on 12 toxigenic strains of Fusarium graminearum isolated from barley and wheat. The susceptible metabolic pathways were assessed through the determination of structural compounds (glucosamine and ergosterol) and enzyme activity of the microorganisms' primary metabolism. The results indicate that phenolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural antifungal agent; Structural compounds; Enzymes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000500012
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Parboiled rice: chemical composition and the occurrence of mycotoxins Ciênc. Tecnol. Aliment.
Dors,Giniani Carla; Bierhals,Vânia da Silva; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the incidence of aflatoxin B1 (AFB B1), deoxynivalenol (DON), ochratoxin A (OTA), and zearalenone (ZEA) in parboiled rice with respect to its chemical composition. Eight lots from five different brands of parboiled rice were collected in four samplings, at different seasons, until the amount of 32 lots. It was observed that: DON was present in 22% of the samples (from 180 to 400 ppb); ZEA in 19% (from 317 to 396 ppb); OTA in 12.5% (from 13 and 26 ppb); and AFB B1 in 9% (from 11 to 74 ppb). The results of the chemical composition were not different from those previously mentioned in the literature concerning parboiled rice. The ash and phenol levels in the contaminated parboiled rice samples suggested that those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deoxynivalenol; Ochratoxin; Physical-chemical characterization; Zearalenone.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100025
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Influência das condições de parboilização na composição química do arroz Ciênc. Tecnol. Aliment.
Dors,Giniani Carla; Pinto,Renata Heidtmann; Badiale-Furlong,Eliana.
O objetivo deste trabalho foi avaliar a influência de diferentes tempos de maceração e autoclavagem, durante o processo de parboilização, na composição química do arroz parboilizado. Uma amostra de arroz verde, com casca e seca, foi submetida ao processo de parboilização segundo um planejamento fatorial 2² com ponto central, tendo como variáveis o tempo de maceração (4, 5 e 6 horas) e o tempo de autoclavagem (15, 22.5 e 30 minutos). Após a secagem, a amostra foi beneficiada em miniengenho, separando-se a casca juntamente com o farelo e o endosperma amiláceo. Todas as porções foram trituradas e peneiradas, recolhendo-se as porções que passaram através de 0,5 mm para serem caracterizadas quanto aos teores de umidade, cinzas, proteínas, fibras, amilose e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arroz parboilizado; Composição química; Tempo de maceração; Tempo de autoclavagem.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000100034
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Deoxynivalenol (DON) degradation and peroxidase enzyme activity in submerged fermentation Ciênc. Tecnol. Aliment.
Garda-Buffon,Jaqueline; Kupski,Larine; Badiale-Furlong,Eliana.
This work aims to evaluate deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryzae in a submerged fermentation system and to correlate it to the activity of oxydo-reductase enzymes. The submerged medium consisted of sterile distilled water contaminated with 50 μg of DON and 4 × 10(6) spore.mL-1 inoculum of Aspergillus oryzae and Rhizopus oryzae species, respectively in each experiment. Sampling was performed every 24 hours for monitoring the peroxidase specific activity, and every 48 hours for determining mycotoxin levels. Results showed that the fungi species were able to decrease DON levels as the peroxidase activity increased. The 48 hours fermentation interval presented the highest peroxidase specific activity (ΔABS/minute.μg.protein-1),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxin; Peroxidase enzyme; Degradation; Fermentation; Fungal species.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100030
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Mycobiota and occurrence of Fumonisin B1 in wheat harvested in Southern Brazil Ciência Rural
Mendes,Gabriela da Rocha Lemos; Reis,Tatiana Alves dos; Corrêa,Benedito; Badiale-Furlong,Eliana.
Although fumonisin B1 (FB1) is a mycotoxin poorly studied in wheat, the Fusarium genus fungi have been frequently detected in this cereal, especially in subtropical regions such as southern Brazil, thus studies on their occurrence are required. This study evaluated the mycobiota, water activity and the presence of FB1 in 11 wheat cultivars grown in the northeastern and northwestern of Rio Grande do Sul. The mycobiota was identified by morphological and molecular techniques and FB1 was determined by liquid chromatography with fluorescence detection (HPLC-FL). Microbial counts lower than 103CFU were found in the samples, and the fungi genera Phoma, Cladosporium and Fusarium were the most frequent. Despite this, 54% of the samples were contaminated with FB1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium; FB1; Triticum aestivum L..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601050
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Common resistance to Fusarium head blight in Brazilian wheat cultivars Scientia Agricola
Mendes,Gabriela da Rocha Lemos; Ponte,Emerson Medeiros Del; Feltrin,Ana Carla; Badiale-Furlong,Eliana; Oliveira,Antônio Costa de.
ABSTRACT: Mycotoxin levels in Fusarium head blight (FHB) infections can be difficult to quantify. The relationship between mycotoxin and disease is not consistent and it is not clear if wheatpathogen interaction is of significance in regions where more than one Fusarium species with distinct trichothecene production ability co-exists. This study aimed to investigate whether a set of eight Brazilian wheat genotypes, varying in resistance according to classification by the breeder, exhibit a common or differential resistance to Fusarium graminearum (Fgra) (deoxynivalenolproducing) and Fusarium meridionale (nivalenol-producing) (Fmer) using full-spike and central spikelet inoculation (type II resistance). Fgra was generally more aggressive than Fmer based on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visual assessment; Spray-inoculation; Point-inoculation; Mycotoxins.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000500426
Registros recuperados: 12
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