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	Provedor de dados BABT (1) BJM (1) Autor Baig,Shahjahan (2) Aftab,Muhammad Nauman (1) Haq,Ikram-ul (1) Nadeem,Muhammad (1) Qazi,Javed Iqbal (1) Palavra-chave Alkaline protease (1) B. licheniformis (1) Bacillus mutants (1) Bacitracin (1) Dehairing agent (1) Mutagenesis (1) Mutagenic treatments (1) Optimization (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Enhanced production of alkaline protease by a mutant of Bacillus licheniformis N-2 for dehairing BABT Nadeem,Muhammad; Qazi,Javed Iqbal; Baig,Shahjahan. The purpose of the present investigations was to improve the yield of alkaline protease for leather dehairing by subjecting the indigenous proteolytic strain Bacillus licheniformis N-2 to various mutagenic treatments viz. UV irradiations, NTG (N-methyl-N-nitro-N-nitrosoguinidine) and MMS (methyl methane sulfonate). After screening on skim milk agar plates, a total of nine positive mutants were selected for shake flask experiments. Among these, the best proteolytic mutant designated as UV-9 showed 1.4 fold higher alkaline protease activity in preoptimized growth medium than the parent strain. The fermentation profile and kinetic parameters such u(h-1), Yp/s, Yp/x, Yx/s, q s, Qs, q p and Qp also indicated the superiority of the selected mutant UV-9 for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus mutants; Mutagenic treatments; Alkaline protease; Dehairing agent. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500003 Systematic mutagenesis method for enhanced production of bacitracin by Bacillus licheniformis mutant strain UV-MN-HN-6 BJM Aftab,Muhammad Nauman; Haq,Ikram-ul; Baig,Shahjahan. The purpose of the current study was intended to obtain the enhanced production of bacitracin by Bacillus licheniformis through random mutagenesis and optimization of various parameters. Several isolates of Bacillus licheniformis were isolated from local habitat and isolate designated as GP-35 produced maximum bacitracin production (14±0.72 IU ml-1). Bacitracin production of Bacillus licheniformis GP-35 was increased to 23±0.69 IU ml-1 after treatment with ultraviolet (UV) radiations. Similarly, treatment of vegetative cells of GP-35 with chemicals like N-methyl N'-nitro N-nitroso guanidine (MNNG) and Nitrous acid (HNO2) increased the bacitracin production to a level of 31±1.35 IU ml-1 and 27±0.89 IU ml-1 respectively. Treatment of isolate GP-35 with... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mutagenesis; Bacitracin; B. licheniformis; Optimization. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100009 Registros recuperados: 2 Primeira ... 1 ... Última

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