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Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) BABT
Klemz,Claudio; Salvo,Ligia Maria; Bastos Neto,Jayme da Cunha; Bainy,Afonso Celso Dias; Assis,Helena Cristina da Silva de.
Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochrome P450; Fish; Ancistrus multispinis; Biomarker.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015
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Effects of aerial exposure on antioxidant defenses in the brown mussel Perna perna BABT
Almeida,Eduardo Alves de; Bainy,Afonso Celso Dias.
Investigations were carried out to evaluate the antioxidant defenses in digestive gland of mussels Perna perna held in air for 4 hours, exposed to air for 4 hours followed by submersion in water for 30 minutes, and constantly submerged for 4.5 hours. No differences were observed in CAT and GPx activities and in the levels of total GSH. Mussels exposed to air had significantly higher SOD activity, possibly related to a preparative mechanism of defense against oxidative stress during reoxygenation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Hypoxia; Mussel; Perna perna; Aerial exposure.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300007
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