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Interference of a commercial catalase preparation in laccase and peroxidase activities BABT
Ballaminut,Nara; Yamanaka,Renata; Machado,Katia Maria Gomes.
The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidase; Fungi; Basidiomycetes; Hydrogen peroxide; Enzymatic inhibition.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500017
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Physiological Characterization of Fungal Inoculum for Biotechnological Remediation of Soils BABT
Ballaminut,Nara; Machado,Kátia Maria Gomes; Oliveira,Luisa Helena dos Santos; Matheus,Dácio Roberto.
The aim of this work was to study the bioremediating potential of Lentinus crinitus CCIBt2611 according to the physiological condition of the inoculum. Inoculum was prepared using sugarcane ground husk (C:N 90), at several physiological ages and applied in soil contaminated with pentachlorophenol. The inoculum's potential was assessed by evaluating the mycelium's vigor at soil's colonization, determination of peroxidase and phenoloxidase activities, in vitro degradation of Remazol Brilliant Blue R and in vivo degradation of pentachlorophenol. The results showed that the assessed parameters were relevant to identify the quality of the inoculum. For L. crinitus, 10 day old inoculum showed good soil-colonization speed with significant enzymatic activities,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Manganese-dependent peroxidase; Organochlorine degradation; Fungal inoculum quality; Bioremediation; Environmental sanitization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400561
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Characterization of fungal inoculum used in soil bioremediation BJM
Ballaminut,Nara; Matheus,Dácio R..
Studies have indicated the capacity of basidiomycetes to degrade recalcitrant organopollutants. However, the age of the fungal inoculum to obtain a more effective degradation has not been defined. The criterion used is total colonization of the substrate. Psilocybe castanella CCB444 and Lentinus crinitus CCB274 have been evaluated in soils containing hexachlorobenzene. In the present study, the physiological conditions of the fungal inocula were characterized on solid substrate (sugarcane bagasse, starch and soy flour). Colonization of the substrate, loss of organic matter, pH variation, organic carbon, total nitrogen, fungal biomass and enzymatic activity were evaluated over 30 days of incubation. Colonization of the substrate was almost complete after 20...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ligninolytic enzymes; Basidiomycetes; Fungal inoculum; Ergosterol.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200011
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