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| Olmos-Zúãiga,J.R.; Jasso-Victoria,R.; Díaz-Martínez,N.E.; Gaxiola-Gaxiola,M.O.; Sotres-Vega,A.; Heras-Romero,Y.; Baltazares-Lipp,M.; Baltazares-Lipp,M.E.; Santillán-Doherty,P.; Hernández-Jiménez,C.. |
| Various methods are available for preservation of vascular grafts for pulmonary artery (PA) replacement. Lyophilization and cryopreservation reduce antigenicity and prevent thrombosis and calcification in vascular grafts, so both methods can be used to obtain vascular bioprostheses. We evaluated the hemodynamic, gasometric, imaging, and macroscopic and microscopic findings produced by PA reconstruction with lyophilized (LyoPA) grafts and cryopreserved (CryoPA) grafts in dogs. Eighteen healthy crossbred adult dogs of both sexes weighing between 18 and 20 kg were used and divided into three groups of six: group I, PA section and reanastomosis; group II, PA resection and reconstruction with LyoPA allograft; group III, PA resection and reconstruction with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary artery graft; Cryopreservation; Lyophilization; Blood vessel bioprosthesis; Prosthesis design; Vascular procedures. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200701 |
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| Sotres-Vega,A.; Baltazares-Lipp,M.; Villalba-Caloca,J.; Gaxiola-Gaxiola,M.O.; Santibañez-Salgado,J.A.; Olmos-Zúñiga,J.R.; Jasso-Victoria,R.. |
| Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tracheal cryopreservation; Epithelium; Caspase-3. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006 |
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