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| Oliveira,Renata Cabrera de; Bando,Érika; Machinski Junior,Miguel. |
| The aim of this work was to optimize and validate, by intralaboratorial procedures, a method for the determination of patulin in grapes by thin-layer chromatography. The steps of extraction, cleanup, detection and quantification were optimized. For the validation of the method, recovery assays with standard solutions and artificially contaminated samples were carried out. The mean recovery and the variation coefficient were 65.4% and 7.58%, respectively. The optimized conditions were: 50 mL of grape juice, three extraction stages (with 100 mL of ethyl acetate in the first stage and 50 mL in second and third stages), and 100 µL of ethanol to solubilize the extract. The solvent-system used was toluene - ethyl acetate - formic acid (6:3:1), and 0.5% MBTH in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Patulin; Mycotoxins; Grape; Thin-layer chromatography. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200023 |
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| Santos,Alini Cristini dos; Souza,Aline Amenencia de; Silva,Milena Veronezi; Nerilo,Samuel Botião; Souza,Alexandra Perdigão Maia de; Bando,Érika; Machinski Junior,Miguel. |
| ABSTRACT: Peanuts are a nutritious food consumed worldwide. Some species of the genus Aspergillus, producers of aflatoxins, colonize peanuts. Human exposure to aflatoxins occurs by ingesting contaminated foods. The objective of this paper was to assess the occurrence and dietary exposure of the population to aflatoxins in peanuts commercially available in Maringa, Brazil, from May 2013 to April 2017. A total of 104 peanut samples were evaluated by thin-layer chromatography and confirmed by derivatization with trifluoroacetic acid. The contamination rate detected was 24.0% in average concentration of 13.4µg/kg. Twenty positive samples (19.2%) exceeded the maximum level permitted in Brazil for the sum of four aflatoxins. Estimated probable daily intake was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Aspergillus flavus; Peanuts; Risk assessment; Public health. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600750 |
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