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	Provedor de dados BJM (1) Autor Barardi,Célia R.M. (1) Simões,Claúdia M.O. (1) Sincero,Thaís C.M. (1) Vinatea,Cecília E.B. (1) Palavra-chave Bioaccumulation (1) Cell culture (1) Oysters (1) Poliovirus (1) RT-PCR (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2006 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Detection of poliovirus type 2 in oysters by using cell culture and RT-PCR BJM Vinatea,Cecília E.B.; Sincero,Thaís C.M.; Simões,Claúdia M.O.; Barardi,Célia R.M.. Shellfish are readily contaminated with viruses present in water containing sewage due to the concentrating effect of filter feeding. Enteroviruses are generally used as a model for the detection of viruses from shellfish due to their public health significance. In the present work, oysters were placed in glass aquaria containing seawater plus unicellular algae. Two experiments were performed: 1) oysters bioaccumulating four different poliovirus type 2 concentrations: 5 x 10(4), 2.5 x 10(4), 5 x 10³ and 5 x 10² PFU/mL during 20h; 2) oyster tissues directly inoculated with 6.0 x 10(5) and 1.0 x 10(5) PFU/mL. After viruses seeding, tissue samples were processed by an adsorption-elution-precipitation method. Positive controls were performed by seeding 6.0 x... Tipo: Info:eu-repo/semantics/article Palavras-chave: Oysters; Poliovirus; Bioaccumulation; Cell culture; RT-PCR. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100012 Registros recuperados: 1 Primeira ... 1 ... Última

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