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Wolbachia spp. interfere na detecção molecular de Ehrlichia canis e Anaplasma platys em cães com microfilaremia Arq. Bras. Med. Vet. Zootec.
Xavier,M.S.; Fonseca,C.N.; Ferreira,R.F.; Castro,T.X.; Neves,F.P.G.; Barbosa,A.V.; Cerqueira,A.M.F.; Almosny,N.R.P..
This report aimed to study the interference in molecular testing for Ehrlichia canis and Anaplasma platys in blood of 155 dogs from the coastal region of Rio de Janeiro. Five Anaplasmataceae positive samples but negative for E. canis and A. platys, from microfilaremic animals, were chosen for sequencing. These sequences, when compared to Gen et Bank database, showed 88% to 100% similarity with Wolbachia spp. denoting an interference in the detection of DNA from other members of Anaplasmataceae, possibly due to a high concentration of Wolbachia spp. DNA.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cão; Erlichiose; Sequenciamento; Microfilarêmico; PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401197
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Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners BJM
Carvalho,A.C.; Barbosa,A.V.; Arais,L.R.; Ribeiro,P.F.; Carneiro,V.C.; Cerqueira,A.M.F..
Abstract Antimicrobial resistance in Escherichia coli isolated from pet dogs can be considered a potential threat of infection for the human population. Our objective was to characterize the resistance pattern, extended spectrum beta-lactamase production and genetic relatedness of multiresistant E. coli strains isolated from dogs (n = 134), their owners (n = 134), and humans who claim to have no contact with dogs (n = 44, control), searching for sharing of strains. The strains were assessed for their genetic relatedness by phylogenetic grouping and pulsed-field gel electrophoresis. Multiresistant E. coli strains were isolated from 42 (31.3%) fecal samples from pairs of dogs and owners, totaling 84 isolates, and from 19 (43.1%) control group subjects. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Antimicrobial resistance; Dogs; Genetic relatedness.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100150
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