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Reinhardt,Érica. L.; Ramos,Patrícia L.; Manfio,Gilson P.; Barbosa,Heloiza R.; Pavan,Crodowaldo; Moreira-Filho,Carlos A.. |
Fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. Ability to fix nitrogen was verified by the acetylene reduction assay. All potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifH probe derived from Azospirillum brasilense. The strains were characterized by RAPD, ARDRA and 16S rDNA sequence analysis. RAPD analyses revealed 8 unique genotypes, the remaining 6 strains clustered into 3 RAPD groups, suggesting a clonal origin. ARDRA and 16S rDNA sequence analyses allowed the assignment of 13 strains to known groups of nitrogen-fixing bacteria, including organisms from the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endophytic bacteria; Diazotrophs; Selective isolation; Molecular systematics. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300002 |
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