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Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimários ovinos Ciência Rural
Bergstein-Galan,Tácia Gomes; Weiss,Romildo Romualdo; Barbosa,Thiago Salvati Rodrigues; Kozicki,Luiz Ernandes; Bicudo,Sony Dimas.
ABSTRACT: Preservation and use of spermatozoa that have been recovered after death can extend the use of genetically superior animals. The objective of this study was to evaluate the maximum period for which ovine spermatozoa could be successfully stored in refrigerated dilution medium post-mortem, with or without added seminal plasma. Three samples of spermatozoa collected in an artificial vagina from 10 rams, or from the tails of four epididymes from the same rams at the time of death (G0) and six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours after death were used. After recovery, the spermatozoa were refrigerated at 5°C in either control medium (CM) or control medium plus 20%homologous seminal plasma (SP) and evaluated for 72 hours...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Refrigeration; Epididymis; Post mortem.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000800700
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