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	Provedor de dados BJM (3) Autor Barbosa-Tessmann,Ione Parra (3) Almeida-Ferreira,Giovanna Caputo (1) Biazio,Gleison Ricardo de (1) Castro,Fausto Fernandes de (1) Leite,Gabriela Guimarães Sousa (1) Machinski Junior,Miguel (1) Pozza,Magali Soares dos Santos (1) Santos,Fabiane Cristina dos (1) Santos,Geraldo Tadeu dos (1) Sega,Rose (1) Tessmann,Dauri José (1) Variane,Augusto Carlos Favaretto (1) Mais... Palavra-chave Aspergillus (1) Corn (1) Dairy (1) Feed (1) Fusarium graminearum (1) Galactose oxidase (1) GaoA gene (1) Molecular identification (1) Mycotoxins (1) Thin-layer chromatography (1) Wheat (1) Zearalenone (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2018 (1) 2013 (1) 2008 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A new PCR approach for the identification of Fusarium graminearum BJM Biazio,Gleison Ricardo de; Leite,Gabriela Guimarães Sousa; Tessmann,Dauri José; Barbosa-Tessmann,Ione Parra. The main objective of this work was to develop a PCR protocol for the identification of Fusarium graminearum, based on a pair of primers targeted to a segment of the 3' coding region of the gaoA gene that codes for the enzyme galactose oxidase (GO). This region has low homology with the same region of GO genes from other fungi. Genomic DNA from 17 strains of Fusarium spp. isolated from diseased cereals, from several other Fusarium species, and from other fungi genera was analyzed in a PCR assay using this primer set. The 17 strains of Fusarium spp. were also analyzed for the GO enzyme production in submerse fermentation in a new formulated liquid medium. All strains that were morphologically and molecularly identified as F. graminearum were able to secrete... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium graminearum; Galactose oxidase; GaoA gene; Molecular identification. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300028 The occurrence of aflatoxigenic Aspergillus spp. in dairy cattle feed in Southern Brazil BJM Variane,Augusto Carlos Favaretto; Santos,Fabiane Cristina dos; Castro,Fausto Fernandes de; Barbosa-Tessmann,Ione Parra; Santos,Geraldo Tadeu dos; Pozza,Magali Soares dos Santos. ABSTRACT The presence of mycotoxins or related fungi in animal feed is a major problem for animal and human health. Silage and concentrated feed samples were collected from 21 dairy farms in the Western part of Paraná state in Southern Brazil. Water activity and pH of all samples were measured, and each sample was analyzed to check for the presence of aflatoxigenic Aspergillus. Water activity was observed to be lower in the concentrated feed samples. The pH was lower in the silage samples, indicating fermentation processes. Two silage samples and four concentrated feed samples were contaminated with Aspergillus spp. Seven isolates of Aspergillus spp. were obtained and their potential to produce aflatoxins was evaluated. Four of the isolates, two from the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Feed; Dairy; Aspergillus. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400919 Occurrence of zearalenone in wheat- and corn-based products commercialized in the State of Paraná, Brazil BJM Almeida-Ferreira,Giovanna Caputo; Barbosa-Tessmann,Ione Parra; Sega,Rose; Machinski Junior,Miguel. The productivity of wheat and corn crops depends on climatic conditions and resistance against phytopathogenic fungi such as those of the genus Fusarium. Some species of this genus produce zearalenone (ZEA), a mycotoxin with hyperestrogenic effects. The objective of this study was to investigate the presence of ZEA in samples of cracked wheat (n = 109), popcorn (n = 51) and corn grits (n = 50) commercialized in the State of Paraná, Brazil. Commercial samples of each crop were collected between September 2007 and June 2008 and analyzed by thin-layer chromatography. The method used for detection of the mycotoxin in wheat and corn derivatives presented a recovery rate of 94.5% and 99.5%, respectively, detection limit of 40 μg.kg-1 and quantification limit of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat; Corn; Zearalenone; Thin-layer chromatography; Mycotoxins. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200005 Registros recuperados: 3 Primeira ... 1 ... Última

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