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	Provedor de dados BJMBR (3) Autor Barbuto,J.A.M. (3) Russo,M. (2) Chaui-Berlinck,J.G. (1) Jancar,S. (1) Nascimento,F.R.F. (1) Ribeiro-Dias,F. (1) Santos,B.C. (1) Schor,N. (1) Starobinas,N. (1) Timenetsky,J. (1) Mais... Palavra-chave Cytokine (1) Cytotoxicity (1) Limiting dilution assay: accuracy (1) Macrophages (1) Membrane-anchored tumor necrosis factor (1) Mycoplasma (1) Nitric oxide (1) Number of (1) Priming (1) Relative error (1) Stress response (1) Transcriptional blockage (1) Translational control (1) Tumor cells (1) Wells (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Ano 2003 (1) 2000 (1) 1998 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells BJMBR Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.. Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)... Tipo: Info:eu-repo/semantics/other Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010 A priori estimation of accuracy and of the number of wells to be employed in limiting dilution assays BJMBR Chaui-Berlinck,J.G.; Barbuto,J.A.M.. The use of limiting dilution assay (LDA) for assessing the frequency of responders in a cell population is a method extensively used by immunologists. A series of studies addressing the statistical method of choice in an LDA have been published. However, none of these studies has addressed the point of how many wells should be employed in a given assay. The objective of this study was to demonstrate how a researcher can predict the number of wells that should be employed in order to obtain results with a given accuracy, and, therefore, to help in choosing a better experimental design to fulfill one's expectations. We present the rationale underlying the expected relative error computation based on simple binomial distributions. A series of simulated in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Limiting dilution assay: accuracy; Wells; Number of; Relative error. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800010 Absence of peripheral blood mononuclear cells priming in hemodialysis patients BJMBR Santos,B.C.; Starobinas,N.; Barbuto,J.A.M.; Russo,M.; Schor,N.. As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 µg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokine; Stress response; Translational control; Transcriptional blockage; Priming; Membrane-anchored tumor necrosis factor. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200009 Registros recuperados: 3 Primeira ... 1 ... Última

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