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Use of carbon and nitrogen stable isotopes to study the feeding ecology of small coastal cetacean populations in southern Brazil Biota Neotropica
Hardt,Fernando Augusto Sliva; Cremer,Marta Jussara; Tonello Junior,Antonio José; Bellante,Antonio; Buffa,Gaspare; Buscaino,Giuseppa; Mazzola,Salvatore; Barreto,André Silva; Martinelli,Luiz Antonio; Zuppi,Giovanni Maria.
Samples from individuals of the populations of Sotalia guianensis (Guiana) and Pontoporia blainvillei (Franciscana) dolphins living in the Babitonga Bay estuary (26° 28′ S/48° 50′ W), and samples from individuals of a second population of P. blainvillei from a nearshore area (26° 38′ S/48° 41′ W), were collected and analyzed along with their prey between 2000 and 2006, to determine the carbon and nitrogen stable isotope ratios and to verify differences in their feeding ecology. No differences were found in the median ð15N values of Guiana (15.2‰) and Franciscana (15.9‰) dolphins living in Babitonga Bay, nor of nearshore Franciscana (15.0‰) individuals, suggesting no variation in the trophic level of these populations. However, the lack of more information...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pontoporia blainvillei; Sotalia guianensis; Babitonga Bay; Feeding ecology; Trophic level.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000400090
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Successful amplification of mitochondrial DNA from dentin of the bottlenose dolphin Tursiops truncatus BABT
Weber,Laura Isabel; Luca,Michelle Jaccottet de; Barreto,André Silva; Souza,Tiago Tolentino de.
Twenty-four teeth of Tursiops truncatus from the collection of the Marine Mammals Laboratory (FURG-RS) were analysed for their potential as DNA source for population genetic and taxonomic studies. All teeth were submitted to decontamination, grinding and demineralisation processes. Dentin powder was weighed for DNA extraction, which was evaluated by the amplification of two mitochondrial genes through PCR using universal primers. Cytochrome-b gene was amplified successfully. PCR products showed concentrations of 3-12 ng/µl for dentin powder, which was positively correlated to the quantity of dentin powder used for DNA extraction. To rule out contamination a SSCP was carried out including positive controls, and samples of humans involved in the analysis....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dolphins; Tursiops; Museum specimens; Dentin; Mitochondrial DNA; Cytochrome-b.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100002
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