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| Beloti,Vanerli; Souza,Juliana Aparecida de; Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. |
| Petrifilm EC and HS were compared to the MPN method to determine their efficacy to enumerate total coliforms and E. coli in 145 samples of water (76 water in natura, 69 drinking water). For water in natura, Petrifilm HS and EC showed good correlation with MPN method. In chlorinated water (< 20 col/100 mL and negatives) the agreement was low. For E.coli enumeration, Petrifilm EC showed a good correlation with MPN method. The results indicated that Petrifilm EC and HS are accurate to enumerate coliforms and E. coli in water when the expected counts are higher than 20/100 mL. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Water; Coliforms; Escherichia coli; Petrifilm. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400002 |
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| Nero,Luís Augusto; Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Santana,Elsa Helena Walter de; Pereira,Mykel Steffani; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. |
| The SimPlate TPC CI system is a rapid method to count mesophilic aerobic microorganisms (MAM) in foods, based on the use of resazurine to indicate bacterial growth. Its efficiency in pasteurized milk was evaluated using 142 pasteurized milk samples (38 type A, 43 type B and 61 type C) collected in Londrina, PR. The standard plating method, using Plate Count Agar (PCA) was used for comparison. The plates of both systems were incubated at 35ºC and read after 24h and 48h. The occurrence of false-positive and false-negative wells and the predominant microorganisms in them were also evaluated. The results were compared by simple correlation and mean variance analyses. The correlation (r) and mean variance values were 0.6811 and 0.7583 for the results obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rapid methods; Milk; SimPlate. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100009 |
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| Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Nunes,Maurício Pinto; Santana,Elsa Helena Walter de; Nero,Luís Augusto; Souza,Juliana Aparecida de. |
| Readycult - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult - LMX. The Readycult - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Readycult - LMX; X-GAL; MUG; Milk; Escherichia coli; Total coliforms. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010 |
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| Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Manoel,Aline Villas-Bôas; d'Ovídio,Loredana; Silva,Lívia Cavaletti da; Franco,Bernadette Dora Gombossy de Melo; Beloti,Vanerli. |
| High levels of microbial contamination, commonly found in animal origin foods and food processing environments, are able to hinder the growth of pathogens in these products and interfere in the results of laboratory analyses for detection of these pathogens. With the aim of verifying the possible interference of the autochthonous microbiota encountered in meat and meat products and processing plants over the presence of Listeria spp., 443 samples, collected from 11 meat retail establishments, were submitted to microbiological analysis to determine the levels of mesophilic aerobes, total coliforms and Escherichia coli and the presence of Listeria spp., according to the methodology proposed by the USDA. The results did not show evident interference of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meat; Listeria spp.; Autochthonous microbiota. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400005 |
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