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| Franco,Ana Cláudia; Hübner,Sílvia de Oliveira; Oliveira,Anna Paula de; Batista,Helena B. de C. Ruthner; Roehe,Paulo Michel; Rijsewijk,Franciscus A.M.. |
| Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycoprotein I (gI), glycoprotein E (gE) and membrane protein US9. To construct the deletion mutant of BoHV-5, the flanking regions of all three genes were cloned in a prokaryotic plasmid. This deletion fragment was co-transfected with the viral DNA into bovine cells. Identification of deletion mutants was performed by immunostaining with an anti-gE monoclonal antibody. One of the gE negative viral populations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus 5; BoHV-5; Deletion mutant; Glycoprotein genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400016 |
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