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Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil BJM
Pereira,Joylson de Jesus; Baumworcel,Natasha; Fioretti,Júlia Monassa; Domingues,Cinthya Fonseca; Moraes,Laís Fernandes de; Marinho,Robson dos Santos Souza; Vieira,Maria Clara Rodrigues; Pinto,Ana Maria Viana; de Castro,Tatiana Xavier.
ABSTRACT The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conjunctivitis; FCV genogroup; Household; Molecular characterization; Shelter.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400777
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Feline herpesvirus 1 viral load related to environmental factors in sheltered cats Ciência Rural
Baumworcel,Natasha; Pereira,Joylson de Jesus; Soares,Ana Maria Barros; Souza,Guilherme Nunes; Almosny,Nadia Regina Pereira; Castro,Tatiana Xavier de.
ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection....
Tipo: Info:eu-repo/semantics/article Palavras-chave: FHV-1; Conjunctivitis; Shelter medicine; QPCR; Animal shelter.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454
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