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| Cianciarullo,Aurora M.; Bonini-Domingos,Claudia R.; Vizotto,Luiz D.; Kobashi,Leonardo S.; Beçak,Maria-Luiza; Beçak,Willy. |
| Abstract Two allopatric populations of Brazilian diploid and tetraploid Odontophrynus americanus species complex, both from São Paulo state, had their blood hemoglobin biochemically analyzed. In addition, these specimens were cytogenetically characterized. Biochemical characterization of hemoglobin expression showed a distinct banding pattern between the allopatric specimens. Besides this, two distinct phenotypes, not linked to ploidy, sex, or age, were observed in adult animals of both populations. Phenotype A exhibits dark-colored body with small papillae, ogival-shaped jaw with reduced interpupillary distance and shorter hind limbs. Phenotype B shows yellowish-colored body with larger papillae, arch-shaped jaw with broader interpupillary distance and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anura; Cryptic species; Hemoglobin differentiation; Polyploidy; Whole-genome duplication. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300436 |
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| Araldi,Rodrigo Pinheiro; Assaf,Suely Muro Reis; Carvalho,Rodrigo Franco de; Carvalho,Márcio Augusto Caldas Rocha de; Souza,Jacqueline Mazzuchelli de; Magnelli,Roberta Fiusa; Módolo,Diego Grando; Roperto,Franco Peppino; Stocco,Rita de Cassia; Beçak,Willy. |
| Abstract In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine papillomavirus (BPV); Bovine papillomatosis; Carcinogenesis; Natural history. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100002 |
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| Cianciarullo,Aurora M.; Naoum,Paulo C.; Bertho,Álvaro L.; Kobashi,Leonardo S.; Beçak,Willy; Soares,Maurilio J.. |
| Erythropoietic and hemoglobin DNA transcriptional activities were analyzed in the diploid and the tetraploid Odontophrynus americanus. Flow cytometric analyses of DNA, RNA and mitochondrial contents showed increased genic activity in both diploid and tetraploid animals during erythropoiesis in vivo elicited by pretreatment phenylhydrazine. Generally, higher values were seen in immature tetraploid erythroid cells. On the 10th day of recovery from anemia, large amounts of messenger RNA were found in both specimens. Based on the mitochondrial content, the tetraploid cells had more intense energy metabolism than the diploid cells. Diploid O. americanus had about three times more erythroid cells than tetraploid specimens, indicating that there were differences... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200020 |
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| Góes,Luiz Gustavo Bentim; Freitas,Antonio Carlos de; Ferraz,Oilita Pereira; Rieger,Tania Tassinari; Santos,José Ferreira dos; Pereira,Alexandre; Beçak,Willy; Lindsey,Charles J.; Stocco,Rita de Cassia. |
| The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine Papillomavirus; BPV4; S2 expression cells. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100001 |
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| Freitas,Antonio Carlos de; Carvalho,Claudemir de; Brunner,Olga; Birgel-Junior,Eduardo Harry; Dellalibera,Alice Maria Melville Paiva; Benesi,Fernando José; Gregory,Lilian; Beçak,Willy; Santos,Rita de Cassia Stocco dos. |
| This study has detected BPV-1 DNA sequences in wart, blood and plasma samples collected from animals affected by papillomatosis, suggesting viral presence inside the cell. We sellected an animal in which we could detect BPV-1 DNA sequences in wart, blood, placenta and amniotic liquid samples and her offspring which presented BPV-1 DNA sequences in blood sample collected immediately after birth. These results show a possible vertical transmission of BPV-1. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BPV; Viral vertical transmission; PCR detection. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500026 |
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| Carvalho,Claudemir de; Freitas,Antonio Carlos de; Brunner,Olga; Góes,Luiz Gustavo Bentim; Cavalcante,Andréa Yaguiu; Beçak,Willy; Santos,Rita de Cassia Stocco dos. |
| Papillomaviruses are described selectively infecting epithelial tissues and are associated with many forms of cancer in different species. Considering the widespread dissemination of papillomatosis in livestock, interest is being centred on possible forms of viral transmission and respective mechanisms. In the present study, we report the detection of bovine papillomavirus (BPV) DNA sequences in female reproductive tract tissues, fluids and oocytes from slaughtered bovines not afflicted by cutaneous papillomatosis. BPV-2 DNA sequences were found in ovarian and uterine tissues as well as in oocytes, cumulus cells and uterine flushings. The presence of papillomavirus sequences in reproductive organ tissues and fluids shows that viral infection in organisms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BPV-2; Papillomatosis; Gametes; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500028 |
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