Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJMBR (2) Autor Beaudet,A. (2) Dal-Farra,C. (1) Nouel,D. (1) Stroh,T. (1) Vandenbulcke,F. (1) Vincent,J.-P. (1) Mais... Palavra-chave Internalization (2) Neurotensin (2) Somatostatin (2) Autoradiography (1) Confocal microscopy (1) Endocytosis (1) Neuropeptides (1) Opioids (1) Ultrastructure (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 1998 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Complementarity of radioautographic and immunohistochemical techniques for localizing neuroreceptors at the light and electron microscopy level BJMBR Beaudet,A.. To assess relationships between neuropeptide-binding sites and receptor proteins in rat brain, the distribution of radioautographically labeled somatostatin and neurotensin-binding sites was compared to that of immunolabeled sst2A and NTRH receptor subtypes, respectively. By light microscopy, immunoreactive sst2A receptors were either confined to neuronal perikarya and dendrites or diffusely distributed in tissue. By electron microscopy, areas expressing somatodendritic sst2A receptors displayed only low proportions of membrane-associated, as compared to intracellular, receptors. Conversely, regions displaying diffuse sst2A labeling exhibited higher proportions of membrane-associated than intracellular receptors. Furthermore, the former showed only low... Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurotensin; Somatostatin; Internalization; Neuropeptides; Autoradiography; Ultrastructure. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200005 Fluorescent ligands for studying neuropeptide receptors by confocal microscopy BJMBR Beaudet,A.; Nouel,D.; Stroh,T.; Vandenbulcke,F.; Dal-Farra,C.; Vincent,J.-P.. This paper reviews the use of confocal microscopy as it pertains to the identification of G-protein coupled receptors and the study of their dynamic properties in cell cultures and in mammalian brain following their tagging with specific fluorescent ligands. Principles that should guide the choice of suitable ligands and fluorophores are discussed. Examples are provided from the work carried out in the authors' laboratory using custom synthetized fluoresceinylated or BODIPY-tagged bioactive peptides. The results show that confocal microscopic detection of specifically bound fluorescent ligands permits high resolution appraisal of neuropeptide receptor distribution both in cell culture and in brain sections. Within the framework of time course experiments,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurotensin; Somatostatin; Opioids; Internalization; Endocytosis; Confocal microscopy. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100017 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco