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Registros recuperados: 11
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QTL mapping for resistance to bonamiosis in the European flat oyster Ostrea edulis ArchiMer
Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2...
Tipo: Text Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf
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Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers ArchiMer
Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and...
Tipo: Text Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf
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A preliminary genetic linkage map for the European flat oyster Ostrea edulis L. ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement...
Tipo: Text Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf
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A preliminary genetic linkage map for the European flat oyster Ostrea eduli ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Halley, Chris; Boudry, Pierre; Beaumont, Andy.
The European flat oyster, Ostrea edulis, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and throughout the Mediterranean. The oyster has been a harvested species for at least 6000 years. However, it successively suffered from over harvesting and then from two protozoan diseases during the 1960s and its production has drastically decreased in Europe from that time. Following two generations of selection for tolerance to one of these diseases (bonamiosis), our aim is to optimize this selection with the assistance of molecular markers, that is to say to implement marker-assisted selection. This will enhance the efficiency of selection by reducing the generation time, as the selection on sites cannot be made before the oysters are...
Tipo: Text Palavras-chave: QTLs; Ostrea edulis; Genetic linkage map; Genetic; European flat oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3473.pdf
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Oocyte activation in Mytilus edulis by Crassostrea gigas spermatozoa ArchiMer
Mccombie, Helen; Cornette, Florence; Beaumont, Andy; Boudry, Pierre.
The development of hatchery production techniques for blue mussels is receiving increased research attention in Europe as it could offer an alternative source of spat for the industry ('Blue Seed' Project, Kamermans ICSR 2007). Among the necessary techniques are those of controlled spawning and fertilization, and techniques of triploidisation that can offer increased seasonal availability, yield and/or decreased growing time in the subsequent production stages. In several experiments, Mytilus edulis oocytes, obtained by thermally induced spawning, were fertilized with Mytilus edulis spermatozoa obtained in the same way or by stripping. Parallel treatments examined the effect of mixing Mytilus edulis oocytes with spermatozoa stripped from the Pacific...
Tipo: Text Palavras-chave: Stripping; Spermatozoa; Crassostrea gigas; Mytilus edulis; Ooctye.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3460.pdf
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Establishment of a genetic map in the European flat oyster Ostrea edulis L. ArchiMer
Lallias, Delphine; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic from European coasts, Atlantic and Mediterranean. It has been exploited since Roman times in Europe. But its production decreased from around 20000 tons in the 1950ies to 1500 tons nowadays because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer initiated a program of selection to produce families of oysters tolerant to Bonamia. In this context, a further step is to identify QTLs of resistance to this parasite. Therefore, a genetic map is first now being built. The establishment of a genetic linkage map will represent the basement for the mapping of QTLs, with the ultimate objective to implement marker-assisted selection in O....
Tipo: Text Palavras-chave: Genetic map; Ostrea edulis; Genetic; European flat oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3472.pdf
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Microsatellites development in Ostrea edulis and Mytilus edulis ArchiMer
Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy.
The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of...
Tipo: Text Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf
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Polyploid fish and shellfish: Production, biology and applications to aquaculture for performance improvement and genetic containment ArchiMer
Piferrer, Francesc; Beaumont, Andy; Falguiere, Jean-claude; Flajshans, Martin; Haffray, Pierrick; Colombo, Lorenzo.
Polyploids can be defined as organisms with one or more additional chromosome sets with respect to the number most frequently found in nature for a given species. Triploids, organisms with three sets of homologous chromosomes, are found spontaneously in both wild and cultured populations and can be easily induced in many commercially relevant species of fish and shellfish. The major consequence of triploidy is gonadal sterility, which is of advantage in the aquaculture of molluscs since it can result in superior growth. In fish, the induction of triploidy is mainly used to avoid problems associated with sexual maturation such as lower growth rates, increased incidence of diseases and deterioration of the organoleptic properties. Triploidy can also be used...
Tipo: Text Palavras-chave: GMO; Transgenic containment; Genetic containment; Reproductive containment; Hybridisation; Sterility; Fish; Shellfish; Fish farming; Aquaculture; Tetraploidy; Triploidy; Polyploidy.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6648.pdf
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A first linkage map for the European flat oyster, Ostrea edulis towards the identification of Bonamia resistance QTLs ArchiMer
Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, a genetic map was therefore established as a basis for Quantitative Trait Loci (QTLs) analysis. The reference family was an F2 family coming from a first bi-parental cross between a wild oyster and an individual from the fifth generation inbred line of the Bonamia tolerance selection program. This inbred line had shown no mortality when reared in...
Tipo: Text Palavras-chave: Parasite; Bonamia ostreae; Bonamiosis; QTL; Genetic; Ostrea edulis; Flat oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3459.pdf
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A first genetic linkage map of the European flat oyster, Ostrea edulis : towards the identification of Bonamia resistance QTLs ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Beaumont, Andy.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, the next step is to identify QTLs for resistance to this parasite. A genetic map is therefore being established as a basis for QTL work. The reference family is an F2 family produced by single pair mating of two oysters from the same F1 family. The F1 family was itself produced from a bi-parental cross between a wild oyster and an individual from the...
Tipo: Text Palavras-chave: Genetic linkage map; Parasite; Genetic; QTLs; Resistance; Bonamia ostreae; Ostrea edulis; Flat oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3464.pdf
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A first genetic linkage map of the blue mussel Mytilus edulis ArchiMer
Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy.
The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and...
Tipo: Text Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf
Registros recuperados: 11
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