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Registros recuperados: 13
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In vitro screening of the effect of three glucosinolate derived nitriles on soil-borne fungi Organic Eprints
Bellostas, Natalia; Casanova, Esther; Garcia-Mina, Jose Maria; Sorensen, Jens Christian; Sorensen, Hilmer.
Glucosinolates are allelochemicals present in all plants of the order Capparales that are hydrolysed by endogenous enzymes (myrosinases) forming a variety of compounds with biological activity. ‘Biofumigation’ is the term used to describe the effect of these compounds on soil-borne pathogens and it has normally been attributed to isothiocyanates. At acidic pH and in the presence of redox co-factors such as glutathione, glucosinolate hydrolysis yields also nitriles, which are more hydrophilic and stable than isothiocyanates. Three nitriles (allyl-, benzyl- and phenethyl cyanide) were tested against soil borne fungi of economic importance: Aphanomyces euteiches var. pisi, Gaeumannomyces graminis var. tritici and Verticillium dahliae. The nitriles were...
Tipo: Conference paper, poster, etc. Palavras-chave: Crop health; Quality; Protection.
Ano: 2006 URL: http://orgprints.org/9261/1/9261.pdf
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Influence of pH and type of myrosinase complex on the products obtained in the myrosinase catalysed hydrolysis of glucosinolates – a MECC study Organic Eprints
Bellostas, Natalia; Sørensen, Jens C.; Sørensen, Hilmer.
Environmental conditions, e.g. pH and the presence of Fe2+ are well known factors that influence the product profile of the myrosinase catalysed hydrolysis of glucosinolates. Depending on the plant genera, the species and tissue of origin myrosinase isoenzymes (thioglucohydrolase EC 3.2.1.147) have different characteristics in terms of MW, subunit composition and pI. However, the influence of these parameters on the outcome of glucosinolate hydrolysis has not been traditionally studied, which hinders the full exploitation of the catalytic potential of these enzymes. In the present experiments the effect of myrosinase type on the products obtained in the hydrolysis of glucosibarin was studied by MECC using two B. carinata myrosinase preparations differing...
Tipo: Conference paper, poster, etc. Palavras-chave: Crop health; Quality; Protection Food security; Food quality and human health.
Ano: 2007 URL: http://orgprints.org/11399/1/11399.pdf
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Glucosinolate profiling of seeds and sprouts of B. oleracea varieties used for food Organic Eprints
Bellostas, Natalia; Kachlicki, Piotr; Sørensen, Jens Christian; Sørensen, Hilmer.
Consumption of Brassica vegetables is related to a decreased incidence of certain cancer forms, and it has been linked to the presence of glucosinolates in these vegetables. After ripe seeds, Brassica sprouts contain the highest concentration of these compounds and are therefore a good source of glucosinolates for chemoprotection. In the present experiments, the content and distribution of glucosinolates in five varieties of B. oleracea (white cabbage, red cabbage, Savoy cabbage, broccoli and cauliflower) was determined in ripe seeds and sprouts of up to seven days by High Performance Liquid Chromatography. The type and concentration of individual glucosinolates varied according to B. oleracea variety, plant part and length of the sprouting period....
Tipo: Journal paper Palavras-chave: Food security; Food quality and human health.
Ano: 2007 URL: http://orgprints.org/11395/1/11395.pdf
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Early interference dynamics in intercrops of pea, barley and oilseed rape Organic Eprints
Bellostas, Natalia; Hauggaard-Nielsen, Henrik; Andersen, Mette Klindt; Jensen, Erik Steen.
Intercropping, the practical application of ecological principles such as diversity, interference, competition and coexistence in agriculture, is gaining increasing interest in organic farming. To be able to improve the management of intercrops, a more detailed knowledge is required on aspects of interference between crops. A bioassay and a pot experiment were conducted to assess both the mechanisms and the time of expression of processes involved in the early interference between pea (Pisum sativum L.), barley (Hordeum vulgare L.) and oilseed rape (Brassica napus L.) when grown as intercrops. Conditions of non-limiting nutrient and water supply were chosen to eliminate competition for these growth factors. The bioassay gave no indication of competitive...
Tipo: Journal paper Palavras-chave: Crop combinations and interactions.
Ano: 2003 URL: http://orgprints.org/1450/2/1450.pdf
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Glucosinolate hydrolysis products for weed control Organic Eprints
Bellostas, Natalia; Kudsk, Per; Sorensen, Jens Christian; Sorensen, Hilmer.
Glucosinolates are allelochemicals present in all Brassica plants. Upon hydrolysis by endogenous enzymes they produce a series of biologically active compounds, such as isothiocyanates and their deriva-tives among others. These compounds have marked fungicidal, nematocidal and herbicidal effects and therefore their use as biodegradable natural products for crop protection has attracted much attention in the last years. A number of these compounds, either individually or in combination, were tested against Sinapis alba and Lollium perenne in Petri dishes bio-assays. C50 values as low as 0.7 and 0.2 mM were obtained. This may open the possibility for using glucosinolate hydrolysis products as herbicides.
Tipo: Conference paper, poster, etc. Palavras-chave: Weed management.
Ano: 2006 URL: http://orgprints.org/9266/1/9266.pdf
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Glucosinolate types and concentrations in seedlings of different Brassica species used for food Organic Eprints
Bellostas, Natalia; Kachlicki, Piotr; Sorensen, Jens Christian; Sorensen, Hilmer.
Brassicaceous food crops contain in their tissues different quantities of the glucoside allelochemicals known as glucosinolates (Bellostas et al., 2004; Sørensen, 1990). These compounds are alkyl-N-hydroximine sulphate esters with a β-D-thioglucopyranoside group attached to the hydroximine carbon in Z-configuration relative to the sulphate group (Ettlinger and Kjær, 1968; Kjær, 1960). Glucosinolates are biosynthetically derived from amino acids (Hill et al., 2003) and they occur in all plants of the order Capparales and in some other plants (Bjerg and Sørensen, 1987; Kjær, 1960; Rodman, 1978). These compounds co-occur with myrosinase isoenzymes (Thioglucosidase; EC 3.2.1.147), which catalyze the hydrolysis of the β-D-thioglucopyranoside bond releasing an...
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health.
Ano: 2005 URL: http://orgprints.org/9267/1/9267.pdf
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Qualitative and quantitative evaluation of glucosinolates in cruciferous plants during their life cycles Organic Eprints
Bellostas, Natalia; Sørensen, Jens Christian; Sørensen, Hilmer.
Glucosinolates produced by Brassica species were investigated in relation to biofumigation, a term used to describe the effects some allelochemicals, including glucosinolate derived products, may have on soil-borne pathogens or other herbivores. Four Brassica species of the U-triangle, namely B. nigra (L.) Koch, B. carinata Braun, B. juncea (L.) Czern. and B. rapa L. were compared with respect to their qualitative and quantitative glucosinolate profiles in roots, stems, leaves and reproductive organs. Plants were monitored at four different development stages and the total glucosinolate content as well as their dry matter production as an indication of their potential biomass under field conditions were determined. Glucosinolate levels of up to 120 ìmol g...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Cereals; Pulses and oilseeds Composting and manuring.
Ano: 2004 URL: http://orgprints.org/5611/1/5611.pdf
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Effect of ascorbic acid and glutathione on the production of nitriles by myrosinase Organic Eprints
Bellostas, Natalia; Sorensen, Jens Christian; Sorensen, Hilmer.
Biofumigation is based on the use of glucosinolate-containing plants for the control of soil-borne pest and diseases. Upon tissue damage, glucosinolates are hydrolyzed by endogenous enzymes (myrosinase) and a range of biologically active compounds are formed. Isothiocyanates (ITCs) are the quantitatively dominating products formed at neutral pH. Most of these compounds are volatile and only sparingly soluble in aqueous systems, and depending on the R-group structure and the presence of nucleophiles, further transformation of ITCs occurs. At lower pH and in the presence of certain molecules able to deliver two redox equivalents, the proportion of nitriles increases at the expense of ITC. The effect of ascorbic acid and glutathione on the production of...
Tipo: Conference paper, poster, etc. Palavras-chave: Crop health; Quality; Protection Food security; Food quality and human health.
Ano: 2006 URL: http://orgprints.org/9263/1/9263.pdf
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Nutritional value of cruciferous oilseed crops in relation to profile of accumulated biomolecules with especial regard to glucosinolates transformation products Organic Eprints
Bellostas, Natalia; Bjergegaard, Charlotte; Jensen, Søren K.; Sørensen, Hilmer; Sørensen, Jens C.; Sørensen, Susanne.
Cruciferous oilseed crops accumulate relatively high concentrations of nutritional high quality oil and proteins in their seeds. In addition to these major seed components, their co-occurrence with high concentrations of dietary fibre (DF) and various bioactive components as glucosinolates/glucosinolate products is decisive for the nutritional value of the seed meal or products obtained from it. Depending on structural types and concentration of glucosinolates and glucosinolate derived products, these compounds can be either health beneficial or act as antinutrients. The effects of these components depend, however, strongly on the type of animal and development of the animals fed with the diets based on these compounds. Results from studies based on...
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health.
Ano: 2007 URL: http://orgprints.org/11397/1/11397.pdf
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Intercropping of cereals and grain legumes for increased production, weed control, im-proved product quality and prevention of N –losses in European organic farming systems Organic Eprints
Jensen, Erik Stee; Ambus, Per; Bellostas, Natalia; Boisen, Sigurd; Brisson, Nadine; Corre-Hellou, Guanelle; Crozat, Yves; Dahlmann, Christoph; Dibet, Audry; von Fragstein, Peter; Gooding, Mike; Hauggaard-Nielsen, Henrik; Kasyanova, Elena; Monti, Michele; Pristeri, Aurelio.
– Intercropping of cereals and grain legumes is a technology to enhance biodiversity in organic agro-ecosystems, enhance and stabilize yields and make better use of plant growth resources. Here we report the preliminary results of the EU FP 5 research project “Intercrop” carried out in five European regions. The project demonstrates several significant benefits which could be exploited to a greater degree in European organic farming .
Tipo: Conference paper, poster, etc. Palavras-chave: Crop combinations and interactions.
Ano: 2006 URL: http://orgprints.org/7659/1/template%2Dintercrop.doc
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Micellar electrokinetic capillary chromatography—Synchronous monitoring of substrate and products in the myrosinase catalysed hydrolysis of glucosinolates Organic Eprints
Bellostas, Natalia; Sorensen, Jens Christian; Sorensen, Hilmer.
A micellar electrokinetic capillary chromatography (MECC) method has been developed for monitoring the myrosinase catalysed hydrolysis of 2-hydroxy substituted glucosinolates and the simultaneous formation of the corresponding degradation products (oxazolidine-2-thiones (OZTs) and nitriles). Glucosibarin ((2R)-2-hydroxy-2-phenylethylglucosinolate) was chosen as the model glucosinolate owing to the difficulties in determining hydrolysis rates of this type of substrates in traditional UV-assays. The method was afterwards validated with glucobarbarin ((2S)-2-hydroxy-2-phenylethylglucosinolate) and progoitrin ((2R)-2-hydroxybut-3-enylglucosinolate). Aromatic glucosinolates without a 2-hydroxy group in their side chains, such as glucotropaeolin...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Food security; Food quality and human health.
Ano: 2006 URL: http://orgprints.org/9260/1/9260.pdf
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Glucosinolate hydrolysis compounds for weed control Organic Eprints
Bellostas, Natalia; Nielsen Kudsk, Per; Sørensen, Jens C.; Sørensen, Hilmer.
Glucosinolates are allelochemicals present in all Brassica plants. Upon hydrolysis by endogenous enzymes they produce a series of biologically active compounds, such as isothiocyanates and their deriva-tives among others. These compounds have marked fungicidal, nematocidal and herbicidal effects and therefore their use as biodegradable natural products for crop protection has attracted much attention in the last years. A number of these compounds, either individually or in combination, were tested against Sinapis alba and Lollium perenne in Petri dishes bio-assays. C50 values as low as 0.7 and 0.2 mM were obtained. This may open the possibility for using glucosinolate hydrolysis products as herbicides.
Tipo: Conference paper, poster, etc. Palavras-chave: Crop health; Quality; Protection Weed management.
Ano: 2006 URL: http://orgprints.org/7501/1/Glucosinolates_for_weed_control.pdf
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Processing-bioprocessing of oilseed rape in bioenergy production and value added utilization of remaining seed components Organic Eprints
Bagger, Christian; Bellostas, Natalia; Jensen, Søren K.; Sørensen, Jens C.; Sørensen, Hilmer; Sørensen, Susanne.
Cruciferous oilseed crops accumulate relatively high concentrations of oil, proteins and dietary fibres (DF) in their seeds, in addition to bioactive components as glucosinolates and myrosinase isoenzymes (thioglucohydrolase; EC 3.2.1.147). When mixed in the presence of moisture, myrosinase isoenzymes and associated components transform glucosinolates into various types of products, which reduces the value of the extracted oil and the remaining seed components, as well as producing unwanted environmental effects due to smell and toxicity. This gives a need for special care concerning myrosinase inactivation as the initial step during processing of oilseed rape, including technologies applied for biodiesel/bioenergy production. The myrosinase inactivation...
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health Processing; Packaging and transportation.
Ano: 2007 URL: http://orgprints.org/11400/1/11400.pdf
Registros recuperados: 13
Primeira ... 1 ... Última
 

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