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| Registros recuperados: 12 | |
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| Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Marcia A.F.; Netto,Daisy Pontes; Pinto,José Paes A.N.; Andrade,Nélio José de; Silva,Wladimir P.; Franco,Bernadette D.G.M.. |
| Fluctuations in the Brazilian milk market force small milk producers to find temporary trade alternatives, which include selling raw milk to people who prefer this type of milk rather than heat-processed milk. Considering the importance of these small milk producers to the market and the well-known health risks associated to consumption of raw milk, this study evaluated the microbiological quality and the presence of Listeria monocytogenes, Salmonella spp., chlorine, antimicrobials and insecticides (organophosphates and carbamates) in raw milk produced in 210 small and medium farms located in four important milk-producing Brazilian states (Paraná, São Paulo, Minas Gerais and Rio Grande do Sul). In 66% of the selected farms the milking was manual. In 33% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hazards; Raw milk; Salmonella; Listeria monocytogenes; Hygiene indicators; Insecticides; Antimicrobials. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200007 |
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| Nero,Luís Augusto; Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Santana,Elsa Helena Walter de; Pereira,Mykel Steffani; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. |
| The SimPlate TPC CI system is a rapid method to count mesophilic aerobic microorganisms (MAM) in foods, based on the use of resazurine to indicate bacterial growth. Its efficiency in pasteurized milk was evaluated using 142 pasteurized milk samples (38 type A, 43 type B and 61 type C) collected in Londrina, PR. The standard plating method, using Plate Count Agar (PCA) was used for comparison. The plates of both systems were incubated at 35ºC and read after 24h and 48h. The occurrence of false-positive and false-negative wells and the predominant microorganisms in them were also evaluated. The results were compared by simple correlation and mean variance analyses. The correlation (r) and mean variance values were 0.6811 and 0.7583 for the results obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rapid methods; Milk; SimPlate. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100009 |
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| Beloti,Vanerli; Souza,Juliana Aparecida de; Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. |
| Petrifilm EC and HS were compared to the MPN method to determine their efficacy to enumerate total coliforms and E. coli in 145 samples of water (76 water in natura, 69 drinking water). For water in natura, Petrifilm HS and EC showed good correlation with MPN method. In chlorinated water (< 20 col/100 mL and negatives) the agreement was low. For E.coli enumeration, Petrifilm EC showed a good correlation with MPN method. The results indicated that Petrifilm EC and HS are accurate to enumerate coliforms and E. coli in water when the expected counts are higher than 20/100 mL. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Water; Coliforms; Escherichia coli; Petrifilm. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400002 |
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| Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Manoel,Aline Villas-Bôas; d'Ovídio,Loredana; Silva,Lívia Cavaletti da; Franco,Bernadette Dora Gombossy de Melo; Beloti,Vanerli. |
| High levels of microbial contamination, commonly found in animal origin foods and food processing environments, are able to hinder the growth of pathogens in these products and interfere in the results of laboratory analyses for detection of these pathogens. With the aim of verifying the possible interference of the autochthonous microbiota encountered in meat and meat products and processing plants over the presence of Listeria spp., 443 samples, collected from 11 meat retail establishments, were submitted to microbiological analysis to determine the levels of mesophilic aerobes, total coliforms and Escherichia coli and the presence of Listeria spp., according to the methodology proposed by the USDA. The results did not show evident interference of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meat; Listeria spp.; Autochthonous microbiota. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400005 |
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| Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Nunes,Maurício Pinto; Santana,Elsa Helena Walter de; Nero,Luís Augusto; Souza,Juliana Aparecida de. |
| Readycult - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult - LMX. The Readycult - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Readycult - LMX; X-GAL; MUG; Milk; Escherichia coli; Total coliforms. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010 |
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| Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Márcia Aguiar Ferreira; Netto,Daisy Pontes; Franco,Bernadette Dora Gombossy de Melo. |
| Organofosforados e carbamatos são compostos utilizados no controle de parasitas em animais e podem gerar resíduos nos produtos alimentícios derivados, representando um risco para o consumidor. O presente estudo objetivou pesquisar a presença de resíduos de organofosforados e carbamatos em leite cru produzido em quatro regiões leiteiras no Brasil e verificar se a presença desses compostos teria alguma relação com a ausência de Listeria monocytogenes e Salmonella spp., anteriormente observada nessas amostras. Entre 209 amostras analisadas, a presença de ao menos um desses compostos foi detectada em 196 (93,8%). Para a avaliação da sua interferência na detecção de L. monocytogenes e Salmonella spp., 28 amostras de leite positivas e negativas para esses... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leite; Pesticidas; Organosfosforados; Carbamatos; Listeria monocytogenes; Salmonella spp. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000100035 |
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| Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Monteiro,Alexandre Amorim; Beloti,Vanerli. |
| The microbiological quality of beef and meat products is strongly influenced by the conditions of hygiene prevailing during their production and handling. Without proper hygienic control, the environment in slaughterhouses and butcher shops can act as an important source of microbiological contamination. To identify the main points of microbiological contamination in the beef processing chain, 443 samples of equipment, installations and products were collected from 11 establishments (1 slaughterhouse and 10 butcher shops) located in the state of Paraná, Brazil. The microbiological quality of all the samples was evaluated using Petri dishes to obtain counts of mesophilic aerobes (AC), total coliforms, Escherichia coli (EC), yeasts and molds (YM). The main... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beef; Contamination; Indicator microorganisms; Meat processing; Escherichia coli. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000400028 |
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| Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Márcia de A. F.; Pinto,José Paes de A. N.; Andrade,Nélio José de; Silva,Wladimir Padilha da; Franco,Bernadette D. G. M.. |
| Evidências que o leite produzido e consumido no Brasil nem sempre apresenta a qualidade desejada têm gerado a discussão e desenvolvimento de novas políticas de incentivo à produção leiteira, resultando no desenvolvimento do Programa Nacional de Melhoria da Qualidade do Leite. Em complementação, em 2002 o Ministério da Agricultura publicou a Instrução Normativa 51 (IN51), com importantes inovações em relação à conservação e transporte do leite cru, além de estabelecimento de um padrão de qualidade para esse tipo de leite (10(6) UFC/mL), a ser implantado em diferentes prazos nas diferentes regiões do país, a partir de 2005. O presente trabalho teve como objetivo verificar se o leite cru produzido em quatro áreas de quatro estados produtores de leite no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leite cru; Qualidade; Legislações; IN51. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000100031 |
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| Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Márcia Aguiar Ferreira; Franco,Bernadette Dora Gombossy de Melo. |
| A presença de resíduos de antibióticos em leite pode ter efeitos adversos para a saúde pública e para a indústria de laticínios. O objetivo desse estudo foi verificar a presença de resíduos de antibióticos pelo kit Charm-test™ (principalmente beta-lactâmicos e sulfonamidas) em 210 amostras de leite cru, coletadas em quatro regiões produtoras de leite no Brasil: 47 em Viçosa - MG, 50 em Pelotas - RS, 63 em Londrina PR e 50 em Botucatu - SP. Resíduos de antibióticos foram detectados em 24 amostras (11,4%), sendo 13 (20,6%) da região de Londrina - PR, 4 (8,0%) da região de Botucatu - SP, 4 (8,5%) da região de Viçosa - MG, e 3 (6,0%) da região de Pelotas - RS. Apesar dos resultados obtidos serem considerados qualitativos, ficou evidente que resíduos de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibióticos; Leite; Charm-test™. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000200031 |
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| Silva,Livia Cavaletti Corrêa da; Tamanini,Ronaldo; Pereira,Juliana Ramos; Rios,Edson Antônio; Ribeiro Junior,José Carlos; Beloti,Vanerli. |
| Milk fraud has been a recurring problem in Brazil; thus, it is important to know the effect of most frequently used preservatives and neutralizing substances as well as the detection capability of official tests. The objective of this study was to evaluate the analytical sensitivity of legislation-described tests and nonspecific microbial inhibition tests, and to investigate the effect of such substances on microbial growth inhibition and the persistence of detectable residues after 24/48h of refrigeration. Batches of raw milk, free from any contaminant, were divided into aliquots and mixed with different concentrations of formaldehyde, hydrogen peroxide, sodium hypochlorite, chlorine, chlorinated alkaline detergent, or sodium hydroxide. The analytical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fraud; Formaldehyde; Hydrogen peroxide; Chlorinated compounds; Inhibitors. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901613 |
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| Beloti,Vanerli; Barros,Márcia A.F.; Freitas,Júlio C. de; Nero,Luís A.; Souza,Juliana A. de; Santana,Elsa H.W.; Franco,Bernadette. D.G.M.. |
| 2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. In this study, TTC was added to Plate Count Agar (PCA) for enumeration of microorganisms in thirty four pasteurized milk samples, with the aim to verify the frequency of microorganisms that are unable to reduce TTC. Milk samples were decimally diluted in saline and pour-plated in PCA plus 0.015% TTC. Colonies were counted after 24h and 48 h of incubation at 35oC. From a total of 50,574 colonies, 19,665 (38.88%) did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2; 3; 5-triphenyltetrazolium chloride TTC pasteurized milk microbial counts. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200009 |
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| Registros recuperados: 12 | |
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