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| Beltrão,Nilzane; Furian,Thales Quedi; Souza,Guilherme Fonseca de; Macagnan,Marisa; Fallavena,Luiz César Bello; Canal,Claudio Wageck. |
| Infectious laryngotracheitis virus (ILTV) cause mild to severe respiratory disease in chickens, the purpose of our study being to use Brazilian isolate of ILTV to reproduce ILTV disease in chickens by experimental infection and to compare three diagnostic methods (nested polymerase chain reaction (PCR), virus isolation, histopathology) for detection of ILTV. Forty-eight chickens intratracheally inoculated with ILTV and a further 48 with PBS, showing mild respiratory signs 48 hours post infection (PI) but no signs of infection after day 10 PI. Every 2 days PI, six birds were arbitrarily selected from the control and infected groups, sacrificed and the trachea collected. Both the nested PCR and virus isolation detected the virus from day 2 until day 12 PI.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious laryngotracheitis virus; Avian pathology; Diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500024 |
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