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Development and optimization of a HPLC-RI method for the determination of major sugars in apple juice and evaluation of the effect of the ripening stage Ciênc. Tecnol. Aliment.
Zielinski,Acácio Antonio Ferreira; Braga,Cíntia Maia; Demiate,Ivo Mottin; Beltrame,Flávio Luís; Nogueira,Alessandro; Wosiacki,Gilvan.
The sugars in apple juice prove its authenticity and its sensory and nutritional properties. The aim of this study was to develop and validate a simple analytical method using high performance liquid chromatography with refractive index detection (HPLC-RI) to determinate and quantify the sugars sucrose, D-glucose, D-fructose, and D-sorbitol polyol in apple juices, as well as to analyze the juices from the Fuji suprema and Lis Gala cultivars at three ripening stages. The analytical performance parameters evaluated indicated that the method was specific for the compounds analyzed, and the linearity of the calibration curves of sugars showed high correlation coefficients (close to 1.0). The limits of detection and quantification are consistent with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malus domestica; Soluble carbohydrates; Liquid chromatography; Validation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000100005
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