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| Souza,A.C.M.F.; Souza,D.R.V.; Sanabani,S.S.; Giorgi,R.R.; Bendit,I.. |
| Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR) for accurate quantification of MYCN gene copy number, we evaluated the analytical performance of this method by comparing the results obtained with it for 101 tumor samples of neuroblastoma to that obtained by absolute and relative real-time PCR. Similar results were obtained for 100 (99%) samples, no significant difference was detected between the median log10 MYCN copy number (1.53 by SQ-PCR versus 1.55 by absolute real-time PCR), and the results of the two assays correlated closely (r = 0.8, Pearson... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Neuroblastoma; Semi-quantitative PCR; Real-time PCR; MYCN; Amplification. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900004 |
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| Gonçalves,J.O.; Tannuri,A.C.A.; Coelho,M.C.M.; Bendit,I.; Tannuri,U.. |
| We previously described a selective bile duct ligation model to elucidate the process of hepatic fibrogenesis in children with biliary atresia or intrahepatic biliary stenosis. Using this model, we identified changes in the expression of alpha smooth muscle actin (α-SMA) both in the obstructed parenchyma and in the hepatic parenchyma adjacent to the obstruction. However, the expression profiles of desmin and TGF-β1, molecules known to be involved in hepatic fibrogenesis, were unchanged when analyzed by semiquantitative polymerase chain reaction (RT-PCR). Thus, the molecular mechanisms involved in the modulation of liver fibrosis in this experimental model are not fully understood. This study aimed to evaluate the molecular changes in an experimental model... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpha smooth muscle actin; Desmin; Hepatic fibrogenesis; Selective bile duct ligation; RT-PCR; TGF-β1. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000850 |
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