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Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station ArchiMer
Coussot, Gaelle; Le Postollec, Aurelie; Incerti, Sebastien; Baque, Mickael; Faye, Clement; Vandenabeele Trambouze, Odile; Cottin, Herve; Ravelet, Corinne; Peyrin, Eric; Fiore, Emmanuelle; Vigier, Flavie; Caron, Jerome; Chaput, Didier; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel.
Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to...
Tipo: Text Palavras-chave: Astrobiology; Cosmic rays; Biochip; Aptamers.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00601/71306/69723.pdf
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Photochemistry on the Space Station-Antibody Resistance to Space Conditions after Exposure Outside the International Space Station ArchiMer
Coussot, Gaelle; Le Postollec, Aurelie; Faye, Clement; Baque, Mickael; Vandenabeele-trambouze, Odile; Incerti, Sebastien; Vigier, Flavie; Chaput, Didier; Cottin, Herve; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel.
Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies. Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, with high affinity and specificity. Studying antibody binding performances under space conditions is mandatory to convince space agencies of the adequacy of this promising tool for planetary exploration. To complement previous ground-based experiments on antibody resistance to simulated irradiation, we evaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 mission outside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days of this mission (220 mGy)...
Tipo: Text Palavras-chave: Astrobiology; Cosmic rays; Biochip; Antibody; Planetary exploration.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00601/71305/69721.pdf
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