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A transposable element annotation pipeline and expression analysis reveal potentially active elements in the microalga Tisochrysis lutea ArchiMer
Berthelier, Jeremy; Casse, Nathalie; Daccord, Nicolas; Jamilloux, Veronique; Saint-jean, Bruno; Carrier, Gregory.
Background Transposable elements (TEs) are mobile DNA sequences known as drivers of genome evolution. Their impacts have been widely studied in animals, plants and insects, but little is known about them in microalgae. In a previous study, we compared the genetic polymorphisms between strains of the haptophyte microalga Tisochrysis lutea and suggested the involvement of active autonomous TEs in their genome evolution. Results To identify potentially autonomous TEs, we designed a pipeline named PiRATE (Pipeline to Retrieve and Annotate Transposable Elements, download: https://doi.org/10.17882/51795), and conducted an accurate TE annotation on a new genome assembly of T. lutea. PiRATE is composed of detection, classification and annotation steps. Its...
Tipo: Text Palavras-chave: Transposable elements; Genome assembly; Pipeline; Tool; Annotation; Algae; Haptophyte; Tisochrysis lutea.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00441/55250/56732.pdf
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Implication of the host TGF beta pathway in the onset of symbiosis between larvae of the coral Fungia scutaria and the dinoflagellate Symbiodinium sp (clade C1f) ArchiMer
Berthelier, Jeremy; Schnitzler, Christine E.; Wood-charlson, Elisha M.; Poole, Angela Z.; Weis, Virginia M.; Detournay, Olivier.
Dinoflagellate-cnidarian associations form both the trophic and structural foundation of coral-reef ecosystems. Previous studies have highlighted the role of host innate immunity in regulation of these partnerships. This study reveals the presence of a transforming growth factor beta (TGF beta) in the coral Fungia scutaria that clusters with TGF beta sensu stricto (ss) from other animals. In functional studies of F. scutaria larvae, we show that (1) TGF beta ss mRNA is expressed during early stages of development prior to the onset of symbiosis; (2) apparent interference of the TGF beta pathway impairs the onset of symbiosis; and (3) this effect is associated with an increase of cytotoxic nitric oxide secretion, an immune response. This work highlights the...
Tipo: Text Palavras-chave: Transforming growth factor beta; Cnidarian; Coral bleaching; Dinoflagellate; Symbiodinium; Symbiosis.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00412/52389/56297.pdf
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PiRATE: a Pipeline to Retrieve and Annotate Transposable Elements ArchiMer
Berthelier, Jeremy; Carrier, Gregory.
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00412/52373/53662.pdf
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