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Bigot, Laetitia. |
The pacific oyster Crassostrea gigas exhibits an annual cycle of reproduction, which occurs in the gonadic area, a mix tissue composed of germinal cells and storage cells that regress during gametogenesis. Neuroendocrine regulations implicated in the physiological balance linking these tissues are not well known. Neuropeptide Y/F, which regulates energetic balance in vertebrates and invertebrates, binds G protein-coupled receptors; five related receptors have been identified in C. gigas. They display a differential pattern of expression depending on reproductive stage, and a higher expression in starved animals, compared to fed animals. By reverse endocrinology, no binding of NPY/F to these receptors could be demonstrated, but one of the receptors... |
Tipo: Text |
Palavras-chave: Crassotrea gigas. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00110/22139/19780.pdf |
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Bigot, Laetitia; Beets, Isabel; Dubos, Marie-pierre; Boudry, Pierre; Schoofs, Liliane; Favrel, Pascal. |
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values... |
Tipo: Text |
Palavras-chave: Oyster; Crassostrea gigas; SNPF; Energy metabolism; Reproduction; Starvation. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00207/31777/30188.pdf |
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Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal. |
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the... |
Tipo: Text |
Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf |
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