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| Biller-Takahashi,Jaqueline Dalbello; Takahashi,Leonardo Susumu; Marzocchi-Machado,Cleni Mara; Zanuzzo,Fabio Sabbadin; Sabioni,Rafael Estevan; Urbinati,Elisabeth Criscuolo. |
| The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. hydrophila; Β-glucan; Immune response; Pacu. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001 |
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| Zanuzzo,Fábio Sabbadin; Biller-Takahashi,Jaqueline Dalbello; Urbinati,Elisabeth Criscuolo. |
| This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus) fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus), to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS) only) at 0.1, 0.2, 0.5 and 1 mg/L). Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Brycon amazonicus; Innate immune system; Oxidative burst; White blood cells. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000023 |
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