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| Bollela,V.R.; Sato,D.N.; Fonseca,B.A.L.. |
| Polymerase chain reaction (PCR) has been widely investigated for the diagnosis of tuberculosis. However, before this technique is applied on clinical samples, it needs to be well standardized. We describe the use of McFarland nephelometer, a very simple approach to determine microorganism concentration in solution, for PCR standardization and DNA quantitation, using Mycobacterium tuberculosis as a model. Tuberculosis is an extremely important disease for the public health system in developing countries and, with the advent of AIDS, it has also become an important public health problem in developed countries. Using Mycobacterium tuberculosis as a research model, we were able to detect 3 M. tuberculosis genomes using the McFarland nephelometer to assess... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tuberculosis; PCR. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900003 |
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| Bollela,V.R.; Frigieri,G.; Vilar,F.C.; Spavieri Jr,D.L.; Tallarico,F.J.; Tallarico,G.M.; Andrade,R.A.P.; de Haes,T.M.; Takayanagui,O.M.; Catai,A.M.; Mascarenhas,S.. |
| Mortality and adverse neurologic sequelae from HIV-associated cryptococcal meningitis (HIV-CM) remains high due to raised intracranial pressure (ICP) complications. Cerebrospinal fluid (CSF) high opening pressure occurs in more than 50% of HIV-CM patients. Repeated lumbar puncture with CSF drainage and external lumbar drainage might be required in the management of these patients. Usually, there is a high grade of uncertainty and the basis for clinical decisions regarding ICP hypertension tends to be from clinical findings (headache, nausea and vomiting), a low Glasgow coma scale score, and/or fundoscopic papilledema. Significant neurological decline can occur if elevated CSF pressures are inadequately managed. Various treatment strategies to address... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Brain diseases; Intracranial hypertension; AIDS-related opportunistic infections; Cryptococcus neoformans; Monitoring. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900501 |
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