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| Costa,E.A.; Vasconcelos,A.C.; Bomfim,M.R.Q.; Amorim,H.B.; Lima,G.B.L.; Coelho,F.M.; Resende,M.. |
| A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Bovine herpesvirus 4; Central nervous system; Encephalitis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400006 |
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| Diniz,S.; Sandes,S.H.C.; Bomfim,M.R.Q.; Santos,P.C.; Cruz,F.D.; Moreira,T.F.; Carvalho,M.R.S.; Cisalpino,P.S.; Moreira,E.S. A.. |
| ABSTRACT Bovine digital dermatitis (BDD) is an infectious and contagious disease characterized by ulcerative and proliferative lesions affecting the skin on the bulbs of the heel or the interdigital cleft in dairy cattle, often associated with lameness. Evidences on the etiology of BDD indicate that it is multifactorial, involving environmental factors and multiple bacterial colonization. We isolated and identified microorganisms from BDD biopsy samples obtained from five Holstein Friesian and two Jersey cows by cultivation and molecular identification of bacterial isolates using 16S rRNA gene sequence analysis. We identified six bacterial species: Spirochetes as Treponema pedis and Leptospira broomi/L. fainei, L. licerasiae/L. wolffii; Corynebacterium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine digital dermatitis; Molecular identification; 16S rRNA gene analysis; Treponema; Leptospira. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300559 |
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| Ferreira,C.; Costa,E.A.; França,S.A.; Melo,U.P. de; Drumond,B.P.; Bomfim,M.R.Q.; Coelho,F.M.; Resende,M.; Palhares,M.S.; Santos,R.L.. |
| Em agosto de 2008, um garanhão da raça Mangalarga Marchador, de quatro anos de idade, com histórico clínico de apatia, inapetência e edema de prepúcio e escroto, apresentou, ao exame clínico, exsudato purulento fluindo pelo óstio prepucial, prepúcio e mucosa peniana com inúmeras lesões circulares de bordos elevados e hiperêmicos, centro ulcerado recoberto por material amarelado de aspecto fibrinoso, com distribuição multifocal. Histologicamente, a mucosa peniana apresentou áreas de ulceração associadas a infiltrado inflamatório misto, com necrose multifocal e moderado acúmulo de fibrina, que se estendiam para o tecido conjuntivo adjacente. O diagnóstico morfológico foi de balanopostite ulcerativa fibrino-necrótica multifocal intensa, similar ao encontrado... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Stallion; Equine herpesvirus 3; Erosive-ulcerative skin lesion; Prepucial region. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600035 |
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| Coelho,F.M.; Maia,M.Q.; Luppi,M.M.; Costa,E.A.; Luiz,A.P.M.F.; Ribeiro,N.A.; Bomfim,M.R.Q.; Fonseca,F.G.; Resende,M.. |
| Blood samples from 1,072 domestic cats of nine administrative regions of Belo Horizonte, MG, were collected and tested using PCR nested for the occurrence of feline leukemia virus (FeLV). Overall occurrence was 47.5% (507/1072) being North (68.1%) and East (54.4%) the most prevalent areas. Epidemiological data showed that FeLV infection was very common among examined cats and breed neither gender nor were predisposing factors for FeLV. The results suggest that the agglomeration of a large number of cats in the same environment can be an important factor for the increase in the rate of transmission of this retrovirus among domestic cats in the studied city. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cat; Feline; Leukemia; Retrovirus; PCR-nested. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300037 |
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