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Bioconversion of R-(+)-limonene to perillic acid by the yeast Yarrowia lipolytica BJM
Ferrara,Maria Antonieta; Almeida,Débora S.; Siani,Antonio C.; Lucchetti,Leonardo; Lacerda,Paulo S.B.; Freitas,André; Tappin,Marcelo R.R.; Bon,Elba P.S..
Perillyl derivatives are increasingly important due to their flavouring and antimicrobial properties as well as their potential as anticancer agents. These terpenoid species, which are present in limited amounts in plants, may be obtained via bioconversion of selected monoterpene hydrocarbons. In this study, seventeen yeast strains were screened for their ability to oxidize the exocyclic methyl group in the p-menthene moiety of limonene into perillic acid. Of the yeast tested, the highest efficiency was observed for Yarrowia lipolytica ATCC 18942. The conversion of R (+)-limonene by Y. lipolytica was evaluated by varying the pH (3 to 8) and the temperature (25 to 30 ºC) in a reaction medium containing 0.5% v/v limonene and 10 gµL of stationary phase cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yarrowia lipolytica; Bioconversion; Limonene; Perillic acid.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400008
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Thermal stability of xylanases produced by Aspergillus awamori BJM
Lemos,Judith Liliana Solórzano; Bon,Elba P.S.; Santana,Maria de Fátima Ebole; Pereira Junior,Nei.
The effect of temperature on the activity and stability of endoxylanase and beta-xylosidase from Aspergillus awamori was investigated. The growth of A. awamori in milled sugar cane bagasse produced predominantly extracellular endoxylanase (30 U/ml) and lower amounts of beta-xylosidase (1.3 U/ml). Grown in sugar cane bagasse as the principal carbon source, the microorganism produced a quite stable beta-xylosidase in a temperature range of 35-55°C, but it exhibited a lower thermostable endoxylanase. The thermostability of endoxylanase was enhanced through addition of polyhydric alcohols, mainly 2 M xylitol and sorbitol solutions. Particular stability upon storage (100%) was found for endoxylanase at -4°C for 165 days. Yet for beta-xylosidase, an activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus awamori; Thermostability; Xylanases.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300010
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