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Borcier, Elodie; Charrier, Gregory; Couteau, Jérôme; Maillet, Géraldine; Le Grand, Fabienne; Bideau, Antoine; Waeles, Mathieu; Le Floch, Stephane; Amara, Rachid; Pichereau, Vianney; Laroche, Jean. |
The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (Platichthys flesus) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between... |
Tipo: Text |
Palavras-chave: Platichthys flesus; Estuaries; Biomarkers; Bioenergetics; Polar lipids; Population genetics. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/76981/78238.pdf |
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Borcier, Elodie; Morvezen, Romain; Boudry, Pierre; Miner, Philippe; Charrier, Gregory; Laroche, Jean; Hegaret, Helene. |
Dinoflagellates of the genus Alexandrium are a major cause of harmful algal blooms (HABs) that have increasingly disrupted coastal ecosystems for the last several decades. Microalgae from the genus Alexandrium are known to produce paralytic shellfish toxins (PST) but also bioactive extracellular compounds (BEC) that can display cytotoxic, allelopathic, ichtyotoxic or haemolytic effects upon marine organisms. The objective of this experimental study was to assess the effects of PST and BEC produced by A. minutum upon juvenile great scallops Pecten maximus. Scallops were exposed for one week to two different strains of A. minutum, the first producing both PST and BEC and the second producing only BEC. Escape response to starfish, daily shell growth,... |
Tipo: Text |
Palavras-chave: Harmful Algal Bloom (HAB); Alexandrium minutum; Pecten maximus; Paralytic Shellfish Toxins (PST); Extracellular compounds; Behaviour; Histology. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00368/47878/47893.pdf |
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Borcier, Elodie; Artigaud, Sebastien; Gaillard, Jean-charles; Armengaud, Jean; Charrier, Gregory; Couteau, Jérôme; Receveur, Justine; Ouddane, Baghdad; Diop, Mamadou; Amara, Rachid; Laroche, Jean; Pichereau, Vianney. |
Estuaries are important areas highly vulnerable to anthropogenic pollutions. Therefore, the assessment of estuarine water quality is a major ecological issue. In this study, we sampled juveniles of the European flounder in the “pristine” Canche estuary, and caged them in Canche and in two polluted sites of the Seine estuary, Rouen and Fosse Nord. After one month, the metal and organic pollutants in these sites were assessed, and we evaluated several phenotypic indicators (condition index, RNA/DNA ratios and genotoxicity), and extracted the proteins in fish livers for analysis using a shotgun proteomics approach. The results showed strong modifications in the fish caged in both sites of the Seine estuary, as compared to those caged in Canche. In particular,... |
Tipo: Text |
Palavras-chave: Estuary water quality; Ecotoxicology; Caging; Suess; Fish physiology; Shotgun proteomics. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00509/62067/66247.pdf |
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