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	Provedor de dados PAB (1) Autor Almeida,Weliton Antonio Bastos de (1) Boscariol,Raquel Luciana (1) Mendes,Beatriz Madalena Januzzi (1) Mourão Filho,Francisco de Assis Alves (1) Palavra-chave Breeding methods (1) Citrus sinensis (1) Epicotyls (1) Seedlings (1) Transgenics (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2002 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Agrobacterium-mediated genetic transformation of 'Hamlin' sweet orange PAB Mendes,Beatriz Madalena Januzzi; Boscariol,Raquel Luciana; Mourão Filho,Francisco de Assis Alves; Almeida,Weliton Antonio Bastos de. The development and optimization of efficient transformation protocols is essential in new citrus breeding programs, not only for rootstock, but also for scion improvement. Transgenic 'Hamlin' sweet orange (Citrus sinensis (L.) Osbeck) plants were obtained by Agrobacterium tumefaciens-mediated transformation of epicotyl segments collected from seedlings germinated in vitro. Factors influencing genetic transformation efficiency were evaluated including seedling incubation conditions, time of inoculation with Agrobacterium and co-culture conditions. Epicotyl segments were adequate explants for transformation, regenerating plants by direct organogenesis. Higher percentage of transformation was obtained with explants collected from seedlings germinated in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Epicotyls; Seedlings; Transgenics; Breeding methods. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000700009 Registros recuperados: 1 Primeira ... 1 ... Última

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