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| Bossus, Maryline; Charmantier, Guy; Blondeau-bidet, Eva; Valletta, Bianca; Boulo, Viviane; Lorin-nebel, Catherine. |
| Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW,... |
| Tipo: Text |
Palavras-chave: ClC-3 chloride channel; Na+/K+-ATPase; Dicentrarchus labrax; Osmoregulation; Osmosensing. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00149/26044/25185.pdf |
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