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Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds Rev. bras. sementes
Barrocas,Ellen Noly; Machado,José da Cruz; Almeida,Mirella Figueiró de; Botelho,Luana Silva; Pinho,Édila Vilela de Resende Von.
Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungus; Molecular detection; Stalk and ear rot; Macrospore leaf spot.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
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