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| Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S. |
| As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist... |
| Tipo: Text |
Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf |
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| Hine, P; Bower, S; Meyer, G; Cochennec, Nathalie; Berthe, Franck. |
| An ultrastructural study was carried out on Mikrocytos mackini, the cause of Denman Island disease in Pacific oysters Crassostrea gigas in western Canada. Three forms were identified, quiescent cells (QC), vesicular cells (VC) and endosomal cells (EC), QC occurred in the vesicular connective tissue (VCT), haemocytes (hyalinocytes), adductor and heart myocytes, and extracellularly, They had a central round to ovoid nucleus, <7 cisternae of inactive nuclear membrane-bound Golgi, few vesicles and lysosome-like bodies. VC were rarely extracellular and usually occurred in adductor and heart myocytes, in close association with host cell mitochondria. The contents of the host cell mitochondria appeared to pass through a tubular extension into the cytoplasm of... |
| Tipo: Text |
Palavras-chave: Denman Island disease; Amitochondriate protozoan; Ultrastructure; Crassostrea gigas; Mikrocytos mackini. |
| Ano: 2001 |
URL: https://archimer.ifremer.fr/doc/2001/publication-815.pdf |
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