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| Chacón,JLV; Brandão,PEB; Villarreal,LYB; Gama,NM; Ferreira,AJP. |
| Trachea, lung, and conjunctive samples from 51 commercial layer farms from Bastos region, São Paulo, Brazil, were submitted to nested-PCR and virus isolation in SPF chicken embryos for ILT diagnosis. This region experienced an outbreak characterized by respiratory signs, decrease in egg production and increased mortality. Out of the 51 tested field samples, 23 were positive for ILT by nested-PCR, 22 were positive after the virus isolation, and 24 were positive when both techniques were used. Newcastle disease virus, Avian pneumovirus, or Mycoplasma gallisepticum were not detected. Infectious bronchitis virus was detected in one farm and Mycoplasma synoviae was detected in eight farms. The high incidence of infectious laryngotracheitis virus (ILTV)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious laryngotracheitis; Nested-PCR; Virus isolation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000100009 |
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| Villarreal,LYB; Brandão,PEB; Chacón,JLV; Doretto Junior,L; Ito,N; Gama,NS; Ishizuka,MM; Luchese,A; Buchala,F; Astolfi-Ferreira,CS; Ferreira,AJP. |
| Avian Infectious Laryngotracheitis, caused by Infectious Laryngotracheitis Virus (ILTV), has been reported for decades in Brazilian laying and broiler flocks. More recently, outbreaks have occurred in São Paulo State. This study reports the application of PCR and DNA sequencing targeted to the p32 gene of ILTV using laying chicken samples from Bastos, São Paulo, Brazil. Three out of four field samples were positive by PCR. DNA sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of ILTV. The results definitely confirmed the presence of ILTV in the birds during the outbreak. Further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian infectious laryngotracheitis; Brazil; P32; PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400011 |
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