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Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain Rev. Bras. Ciênc. Avic.
Baratto,CM; Gelinski,JMLN; Bernardi,AZ; Marafon,A; Braun,F.
Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular analysis; Salmonella; Serotypes; Typing.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300003
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