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Sperm and Egg Jelly Coat from Sea Urchin Lytechinus variegatus Collected in Rio de Janeiro Contain Distinct Sialic Acid-Rich Polysaccharides BABT
Valle,Gabrielle M.; Cinelli,Leonardo P.; Todeschini,Adriane R.; Brito-Gitirana,Lycia de; Vilela-Silva,Ana-Cristina E.S.; Mourão,Paulo A.S..
This work found the occurrence of a distinct sialic acid-rich polysaccharide in the sperm surface of the sea urchin Lytechinus variegatus, which differed significantly from a similar molecule found in the egg jelly. The sperm polysaccharide extracted by protease digestion was purified using anion exchange chromatography and characterized using agarose gel electrophoresis, gas chromatography/mass spectrometry and NMR spectroscopy. This polysaccharide was highly sulfated and composed almost exclusively of N-acetylneuraminic acid. In contrast, the sialic acid-rich polysaccharide from the egg jelly was composed of N-glycolylneuraminic acid and contains several other hexoses in its structure. This new molecule could help to characterize in further detail the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sea urchin; Sperm; Egg jelly coat; Fertilization; Sialic acid; Sulfated polysaccharide.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400617
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Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae) Rev. Bras. Zool.
Silva,Henrique Alencar Meira da; Silva-Soares,Thiago; Brito-Gitirana,Lycia de.
ABSTRACT The integuments of ten treefrog species of two genera from Scinaxnae - O. angrensis (Lutz, 1973), O. flavoguttata (Lutz & Lutz, 1939), O. humilis (Lutz & Lutz, 1954), O. perpusilla (Lutz & Lutz, 1939), O. v-signata (Lutz, 1968), Scinax hayii (Barbour, 1909), S. similis (Cochran, 1952), O. trapicheroi (Lutz & Lutz, 1954) and S. x-signatus (Spix, 1824) - were investigated using conventional and histochemical techniques of light microscopy, and polarized light microscopy. All integuments showed the basic structure of the anuran integument. Moreover, the secretory portions of exocrine glands, such as serous merocrine and apocrine glands, were found to be restricted to the spongious dermis. Lipid content occurred together with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian Atlantic forest; Histochemistry; Hylids; Treefrog.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702017000100327
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Histological and immunohistochemical study of the ovaries and oviducts of the juvenile female of Caiman latirostris (Crocodilia: Alligatoridae) Rev. Bras. Zool.
Machado-Santos,Clarice; Santana,Lailah N. de S.; Vargas,Rosilene F.; Abidu-Figueiredo,Marcelo; Brito-Gitirana,Lycia de; Chagas,Maurício A..
ABSTRACT The purpose of this study was to examine the ovaries and the oviduct of juvenile females of the broad-snouted caiman, Caiman latirostris (Daudin, 1802), using light microscopy, histochemical and immunocytochemical techniques. The ovarian cortex was observed to contain groups of germinative cells and abundant previtellogenic follicles at different stages of development. In previtellogenic follicles, the oocyte was surrounded by a single cuboidal layer of granulosa cells, supported by the theca layer. The theca was formed by a concentric layer containing collage nous fibers, predominantly type I, and several smooth muscle fibers. The inner perivitelline layer appeared as a narrow basophilic region between the oocyte and the granulosa. The ovarian...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Female reproductive system; Morphology; Reptiles.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000500395
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