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Registros recuperados: 5
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Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers Genet. Mol. Biol.
Zucchi,Maria Imaculada; Brondani,Rosana Pereira Vianello; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Vencovsky,Roland.
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; Myrtaceae; Cerrado; Tropical tree; Genetic diversity and transferability.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008
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Comparative linkage mapping of Oryza glumaepatula and Oryza sativa interspecific crosses based on microsatellite and expressed sequence tag markers Genet. Mol. Biol.
Rangel,Priscila Nascimento; Brondani,Rosana Pereira Vianello; Coelho,Alexandre Siqueira Guedes; Rangel,Paulo Hideo Nakano; Brondani,Claudio.
Molecular linkage maps representing the rice genome have been an important tool for breeding programs because they allow the elucidation of polygenic traits and are an efficient tool for monitoring wild introgressions in interspecific crosses. Common markers among rice genetic maps are important in defining the homology of chromosomes and the synteny between genomic target regions. We used 148 markers (expressed sequence tags, microsatellites and single nucleotide polymorphisms) to construct a molecular linkage map based on co-dominant markers for an interspecific backcross population using a wild rice (Oryza glumaepatula) from Brazil and performed a comparative analysis with other interspecific maps. The comparative analysis revealed a Spearman...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comparative linkage map; Molecular markers; Oryza glumaepatula; Rice.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400019
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Development of microsatellite markers for the genetic analysis of Magnaporthe grisea Genet. Mol. Biol.
Brondani,Claudio; Brondani,Rosana Pereira Vianello; Garrido,Lucas da Ressurreição; Ferreira,Márcio Elias.
An AG microsatellite-enriched genomic DNA library was constructed for Magnaporthe grisea (anamorph Pyricularia grisea), the causal agent of rice blast. Seventy-two DNA clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new PCR-based molecular markers to be used in genetic studies of the fungus. Twenty-four of these clones were selected to design primer pairs for the PCR amplification of microsatellite alleles. Single spore cultures of M. grisea isolated from rice and wheat in Brazil, Colombia and China were genotyped at three microsatellite loci. Isolates from southern Brazil were predominantly monomorphic at the tested SSR loci, indicating a low level of genetic variability in these samples. However, seven...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400009
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Association mapping for yield and grain quality traits in rice (Oryza sativa L.) Genet. Mol. Biol.
Borba,Tereza Cristina de Oliveira; Brondani,Rosana Pereira Vianello; Breseghello,Flávio; Coelho,Alexandre Siqueira Guedes; Mendonça,João Antônio; Rangel,Paulo Hideo Nakano; Brondani,Claudio.
Association analysis was applied to a panel of accessions of Embrapa Rice Core Collection (ERiCC) with 86 SSR and field data from two experiments. A clear subdivision between lowland and upland accessions was apparent, thereby indicating the presence of population structure. Thirty-two accessions with admixed ancestry were identified through structure analysis, these being discarded from association analysis, thus leaving 210 accessions subdivided into two panels. The association of yield and grain-quality traits with SSR was undertaken with a mixed linear model, with markers and subpopulation as fixed factors, and kinship matrix as a random factor. Eight markers from the two appraised panels showed significant association with four different traits,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Association analysis; Core collection; Genetic structure.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300023
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Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite markers Genet. Mol. Biol.
Brondani,Claudio; Borba,Tereza Cristina Oliveira; Rangel,Paulo Hideo Nakano; Brondani,Rosana Pereira Vianello.
The rice (Oryza sativa) breeding program of the Rice and Bean research center of the Brazilian agricultural company Empresa Brasileira de Pesquisa Agropecuária (Embrapa) is well established and provides new cultivars every year to attend the demand for improved high yielding varieties with tolerance to biotic and abiotic stresses. However, the elite genitors used to compose new populations for selection are closely related, contributing to the yield plateau reached in the last 20 years. To overcome this limit, it is necessary to broaden the genetic basis of the cultivars using diverse germplasm such as wild relatives or traditional varieties, with the latter being more practical because they are more easily crossed with elite germplasm to accelerate the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Genetic resources; Microsatellite markers; Oryza sativa.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400017
Registros recuperados: 5
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