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Couturier, Lydie; Michel, Loic; Amaro, Teresa; Budge, Suzanne M; Da Costa, Elisabete; De Troch, Marleen; Di Dato, Valeria; Fink, Patrick; Giraldo, Carolina; Le Grand, Fabienne; Loaiza, Iván; Mathieu-resuge, Margaux; Nichols, Peter D; Parrish, Christopher C; Sardenne, Fany; Vagner, Marie; Pernet, Fabrice; Soudant, Philippe; Browman, Howard. |
Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA... |
Tipo: Text |
Palavras-chave: Aquatic nutrition; Biomarkers; Fatty acids; Marine and freshwater lipids; Methods; Recommended practices. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00651/76279/77252.pdf |
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Thingstad, T Frede; Browman, Howard. |
In linear food chains, resource and predator control produce positive and negative correlations, respectively, between biomass at adjacent trophic levels. These simple relationships become more complex in food webs that contain alternative food chains of unequal lengths. We have used a “minimum” model for the microbial part of the pelagic food web that has three such food chains connecting free mineral nutrients to copepods: via diatoms, autotrophic flagellates, and heterotrophic bacteria. Trophic cascades from copepods strongly modulates the balance between the three pathways and, therefore, the functionality of the microbial food web in services such as food production for higher trophic levels, DOM degradation, and ocean carbon sequestration. The result... |
Tipo: Text |
Palavras-chave: Arctic Ocean; Bottom-up; Mediterranean Sea; Microbial food web; Nansen Legacy; Top down; Trophic cascades. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00627/73958/73312.pdf |
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