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| Oliveira,Maíra Maciel Mattos de; Brugnera,Danilo Florisvaldo; Alves,Eduardo; Piccoli,Roberta Hilsdorf. |
| An experimental model was proposed to study biofilm formation by Listeria monocytogenes ATCC 19117 on AISI 304 (#4) stainless steel surface and biotransfer potential during this process. In this model, biofilm formation was conducted on the surface of stainless steel coupons, set on a stainless steel base with 4 divisions, each one supporting 21 coupons. Trypic Soy Broth was used as bacterial growth substrate, with incubation at 37 ºC and stirring of 50 rpm. The number of adhered cells was determined after 3, 48, 96, 144, 192 and 240 hours of biofilm formation and biotransfer potential from 96 hours. Stainless steel coupons were submitted to Scanning Electron Microscopy (SEM) after 3, 144 and 240 hours. Based on the number of adhered cells and SEM, it was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria monocytogenes; Biofilm; Biotransfer potential. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100016 |
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| Oliveira,Maíra Maciel Mattos de; Brugnera,Danilo Florisvaldo; Mendonça,Alexandre Tourino; Piccoli,Roberta Hilsdorf. |
| Foram avaliadas condições higiênico-sanitárias de máquinas de moer carne e mãos de manipuladores e observou-se a interferência na qualidade microbiológica da carne moída. Nas máquinas de moer carne e mãos de manipuladores, realizaram-se análises de microrganismos aeróbios mesófilos, fungos filamentosos e leveduras, coliformes totais e termotolerantes, Escherichia coli e estafilococos coagulase positiva. Nenhuma das máquinas enquadrou-se nos padrões da APHA. Para manipuladores, as contagens de aeróbios mesófilos, estafilococos coagulase positiva, fungos filamentosos e leveduras, coliformes totais e termotolerantes apresentaram-se elevadas. Nas amostras de carne inteira e moída, realizaram-se, além das citadas acima, análises de Salmonella sp. aeróbios... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contaminação cruzada; Carne moída; Análise microbiológica. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000600031 |
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| Caixeta,Danila Soares; Scarpa,Thiago Henrique; Brugnera,Danilo Florisvaldo; Freire,Dieyckson Osvani; Alves,Eduardo; Abreu,Luiz Ronaldo De; Piccoli,Roberta Hilsdorf. |
| The biofilm formation of Pseudomonas aeruginosa and Pseudomonas fluorescens on AISI 304 stainless steel in the presence of reconstituted skim milk under different temperatures was conducted, and the potential of three chemical sanitizers in removing the mono-species biofilms formed was compared. Pseudomonas aeruginosa cultivated in skim milk at 28 °C presented better growth rate (10.4 log CFU.mL-1) when compared with 3.7 and 4.2 log CFU.mL-1 for P. aeruginosa and P. fluorescens cultivated at 7 °C, respectively. Pseudomonas aeruginosa formed biofilm when cultivated at 28 °C. However, only the adhesion of P. aeruginosa and P. fluorescens was observed when incubated at 7 °C. The sodium dichloroisocyanurate was the most efficient sanitizer in the reduction of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm; P. aeruginosa; P. fluorescens; Chemical sanitizers. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100021 |
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